Kent Academic Repository

Repository Validation Report

Base URL:  http://kar.kent.ac.uk/cgi/oai2
Sample date:  2017-10-05
Sample size:  100 records harvested

Results

RecordValidation ReportsToggle Raw XML
ID: oai:kar.kent.ac.uk:477
Date: 2015-11-11

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:477</identifier>
      <datestamp>2015-11-11T08:59:25Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D54</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132333030:31323330302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:language>en</dc:language><dc:publisher>Institution of Engineering and Technology</dc:publisher><dc:source>0013-5194</dc:source><dc:subject>T</dc:subject><dc:title>Texture Classification System using Colour Space Fusion</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Chindaro, Samuel</rioxxterms:author><rioxxterms:author>Sirlantzis, Konstantinos</rioxxterms:author><rioxxterms:author>Deravi, Farzin</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2005-05</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1049/el:20050594</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:452
Date: 2016-04-28

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
rioxxterms:version_of_record'DOI not available' is not a valid HTTP URI in rioxxterms:version_of_record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
rioxxterms:version_of_record'DOI not available' is not a valid HTTP URI in rioxxterms:version_of_record
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:452</identifier>
      <datestamp>2016-04-28T12:24:04Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D54:544B</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132333030:31323330302F33</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>This paper describes the design and experimental evaluation of a radiometric instrumentation system that has recently been developed for the measurement of volumetric concentration, velocity and mass flow rate of pneumatically conveyed solids. The system employs ‘micro’ beam collimation of gamma radiation to generate multiple, parallel interrogation beams of small cross-sectional area. This configuration is shown to almost eliminate the geometrical errors associated with more conventional&#13;
divergent-beam interrogation. Experimental results obtained off-line using idealized flow models, and also on-line using a pneumatic conveyor, demonstrate the performance of the system and highlight where further development is needed</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/452/1/Item_452.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Institute of Physics Publishing</dc:publisher><dc:source>0957-0233</dc:source><dc:subject>TK</dc:subject><dc:title>A Parallel-Beam Radiometric Instrumentation System for the Mass Flow Measurement of Pneumatically Conveyed Solids</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Barratt, I.R.</rioxxterms:author><rioxxterms:author>Yan, Yong</rioxxterms:author><rioxxterms:author>Byrne, B.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>DOI not available</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:431
Date: 2015-03-26

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:431</identifier>
      <datestamp>2015-03-26T10:38:39Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D54:544B</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132333030:31323330302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><ali:free_to_read/><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/431/1/431-LockingBW-IEEProc.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>IEEE</dc:publisher><dc:source>IEE Proceedings: Optoelectronics</dc:source><dc:subject>TK</dc:subject><dc:title>Locking Bandwidth Equations for Electrically and Optically Injection-Locked Oscillators</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Wang, X.</rioxxterms:author><rioxxterms:author>Gomes, Nathan J.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004-12</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1049/ip-opt:20040793</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:423
Date: 2017-01-13

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:423</identifier>
      <datestamp>2017-01-13T20:50:38Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The eukaryotic initiation factor 4G (eIF4G) is the core of a multicomponent switch controlling gene expression at the level of translation initiation. It interacts with the small ribosomal subunit interacting protein, eIF3, and the eIF4E/cap-mRNA complex in order to load the ribosome onto mRNA during cap-dependent translation. We describe the solution structure of the complex between yeast eIF4E/cap and eIF4G (393-490). Binding triggers a coupled folding transition of eIF4G (393-490) and the eIF4E N terminus resulting in a molecular bracelet whereby eIF4G (393-490) forms a right-handed helical ring that wraps around the N terminus of eIF4E. Cofolding allosterically enhances association of eIF4E with the cap and is required for maintenance of optimal growth and polysome distributions in vivo. Our data explain how mRNA, eIF4E, and eIF4G exists as a stable mRNP that may facilitate multiple rounds of ribosomal loading during translation initiation, a key determinant in the overall rate of protein synthesis.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/423/1/Gross_et_al_2003%2C_Cell_115%2C_739-750.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Elsevier</dc:publisher><dc:source>0092-8674</dc:source><dc:subject>Q</dc:subject><dc:title>Ribosome loading onto the mRNA cap is driven by conformational coupling between eIF4G and eIF4E</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Gross, John D.</rioxxterms:author><rioxxterms:author>Moerke, Nathan</rioxxterms:author><rioxxterms:author>von der Haar, Tobias</rioxxterms:author><rioxxterms:author>Lugovskoy, A.A.</rioxxterms:author><rioxxterms:author>Sachs, A.B.</rioxxterms:author><rioxxterms:author>McCarthy, John E. G.</rioxxterms:author><rioxxterms:author>Wagner, Gerhard</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2003</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:422
Date: 2016-04-28

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:422</identifier>
      <datestamp>2016-04-28T12:13:47Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F33</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Recent research has revealed a conserved role for the actin cytoskeleton in the regulation of aging and apoptosis among eukaryotes. Here we show that the stabilization of the actin cytoskeleton caused by deletion of Sla1p or End3p leads to hyperactivation of the Ras signaling pathway. The consequent rise in cyclic AMP (cAMP) levels leads to the loss of mitochondrial membrane potential, accumulation of reactive oxygen species (ROS), and cell death. We have established a mechanistic link between Ras signaling and actin by demonstrating that ROS production in actin-stabilized cells is dependent on the G-actin binding region of the cyclase-associated protein Srv2p/CAP. Furthermore, the artificial elevation of cAMP directly mimics the apoptotic phenotypes displayed by actin-stabilized cells. The effect of cAMP elevation in inducing actin-mediated apoptosis functions primarily through the Tpk3p subunit of protein kinase A. This pathway represents the first defined link between environmental sensing, actin remodeling, and apoptosis in Saccharomyces cerevisiae.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/422/1/Gourlay_%26_Ayscough_2006.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Society for Microbiology</dc:publisher><dc:source>0270-7306</dc:source><dc:subject>Q</dc:subject><dc:title>Actin-Induced Hyperactivation of the Ras Signaling Pathway Leads to Apoptosis in Saccharomyces cerevisiae</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Gourlay, Campbell W.</rioxxterms:author><rioxxterms:author>Ayscough, Kathryn R.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2006</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:407
Date: 2015-04-28

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:407</identifier>
      <datestamp>2015-04-28T12:49:24Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48</setSpec>
      <setSpec>7375626A656374733D48:484D</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F35</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:language>en</dc:language><dc:source>Journal of Social Policy</dc:source><dc:subject>H</dc:subject><dc:subject>HM</dc:subject><dc:title>The Silver Age of the Welfare State: Perspectives on Resilience</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Taylor-Gooby, Peter</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2002</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:406
Date: 2015-04-28

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:406</identifier>
      <datestamp>2015-04-28T12:48:42Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48</setSpec>
      <setSpec>7375626A656374733D48:484D</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F35</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Welfare spending in the UK is too low to provide services at the level to which most citizens aspire. The problem is that, although most people state in surveys that they would like to pay higher taxes for better services, politicians from all the main parties generally do not believe that they would put their vote where their mouth is. Advocates of higher spending increasingly retreat to a position of promoting ear-marked taxes for specific, highly favoured services, as in the 2002 Budget plan to finance the cost of improvements in the highly valued NHS through increased National Insurance Contributions. Recent theoretical work further undermines arguments for higher state spending: an important strand in political science argues that trust in state institutions is in decline, and work in sociology claims that citizens are becoming more independent, reflexive and keen to take responsibility for meeting their own needs. This paper uses data from a recent ESRC-financed national survey to examine these arguments. It shows real support for hypothecated taxes for the NHS, and more generalised support for higher taxes for welfare provision. Such support is not undermined by a decline in citizen trust in the welfare state or by a rejection of collective solutions. However, there is little endorsement of hypothecated taxation in other areas, and the use of such measures may encourage citizens in a pick-and-mix approach to welfare services.</dc:description><dc:language>en</dc:language><dc:publisher>CAMBRIDGE UNIVERSITY PRESS</dc:publisher><dc:source>0047-2794 </dc:source><dc:subject>H</dc:subject><dc:subject>HM</dc:subject><dc:title>Paying for 'World Class' Services: A British Dilemma.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Taylor-Gooby, Peter</rioxxterms:author><rioxxterms:author>Hastie, Charlotte L.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2003-04</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1017/S0047279402006992</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:405
Date: 2015-04-28

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:405</identifier>
      <datestamp>2015-04-28T12:48:01Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48</setSpec>
      <setSpec>7375626A656374733D48:484D</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F35</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:language>en</dc:language><dc:source>Journal of Social Policy</dc:source><dc:subject>H</dc:subject><dc:subject>HM</dc:subject><dc:title>Market Means: Welfare Ends: the UK Welfare State Experiment</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Taylor-Gooby, Peter</rioxxterms:author><rioxxterms:author>Larsen, Trine P.</rioxxterms:author><rioxxterms:author>Kananen, Johannes K.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:404
Date: 2015-04-29

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:404</identifier>
      <datestamp>2015-04-29T09:36:41Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48</setSpec>
      <setSpec>74797065733D626F6F6B</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F35</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>This book introduces the concept of new social risks in welfare state studies and explains their relevance to the comparative understanding of social policy in Europe. New social risks arise from shifts in the balance of work and family life as a direct result of the declining importance of the male breadwinner family, changes in the labour market, and the impact of globalization on national policy-making. They differ from the old social risks of the standard industrial life-course, which were concerned primarily with interruptions to income from sickness, unemployment, retirement, and similar issues. New social risks pose new challenges for the welfare policies of European countries, such as the care of children and the elderly, more equal opportunities, the activation of labour markets and the management of needs that arise from welfare state reform, and new opportunities for the coordination of policies at the EU level. The book includes detailed and up-to-date case studies of policy development across these areas in the major European countries. These studies, written by leading experts, are organized in a comparative framework which is followed throughout the book. They highlight the way in which national welfare state regimes and institutional arrangements shape policy-making to meet new social risks. A major feature of this volume is the analysis of developments at the EU level and their interaction with national policies. The EU has been largely unsuccessful in its interventions in old social risk policy, but appears to have more success in its attempts to coordinate policy for new social risks. Experience here may provide lessons for future developments in EU policy-making. The comparative framework of the book seeks to inform an understanding of the development of new social risks in Europe and of the particular political opportunities and challenges that result. It provides an original analysis of pressing issues at the forefront of European welfare policy debate and locates it at the heart of current theoretical debates. </dc:description><dc:language>en</dc:language><dc:publisher>Oxford University Press</dc:publisher><dc:source>9780199267262</dc:source><dc:subject>H</dc:subject><dc:title>New Risks, New Welfare: The Transformation of the European Welfare State</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Taylor-Gooby, Peter</rioxxterms:author><rioxxterms:contributor>Taylor-Gooby, Peter</rioxxterms:contributor><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004-11</rioxxterms:publication_date><rioxxterms:type>Book</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:401
Date: 2016-11-25

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:401</identifier>
      <datestamp>2016-11-25T13:26:21Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F35</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:language>en</dc:language><dc:source>Journal of Housing and Built Environment</dc:source><dc:subject>H</dc:subject><dc:title>Four varieties of comparative analysis</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Pickvance, Chris</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:400
Date: 2016-11-25

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:400</identifier>
      <datestamp>2016-11-25T13:32:12Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48</setSpec>
      <setSpec>74797065733D626F6F6B</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F35</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:language>en</dc:language><dc:publisher>Ashgate</dc:publisher><dc:subject>H</dc:subject><dc:title>Local Environmental Regulation in Post-Socialism: a Hungarian Case Study</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Pickvance, Chris</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2003</rioxxterms:publication_date><rioxxterms:type>Book</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:357
Date: 2015-04-29

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:357</identifier>
      <datestamp>2015-04-29T09:37:05Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48</setSpec>
      <setSpec>7375626A656374733D48:484D</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F34</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:language>en</dc:language><dc:publisher>Nomos</dc:publisher><dc:source>3789074535</dc:source><dc:subject>H</dc:subject><dc:subject>HM</dc:subject><dc:title>Zwischen Gestaltungsanspruch und Strukturvorgaben. Junge Fachkraefte in den ersten Berufsjahren - Erwerbsverlaeufe, Handlungskontexte und biographische Gestaltungsmodi</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Zinn, Jens O.</rioxxterms:author><rioxxterms:contributor>Taylor-Gooby, Peter</rioxxterms:contributor><rioxxterms:contributor>Zinn, Jens O</rioxxterms:contributor><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:356
Date: 2015-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:356</identifier>
      <datestamp>2015-04-27T13:52:19Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48</setSpec>
      <setSpec>7375626A656374733D48:484D</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F34</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>As a result of continuous social change, it seems more and more difficult today to produce biographical certainty in the sense of clear expectations and the shaping of one's own life course. What was previously taken for granted is being transformed into (real or apparent) decisions by means of social individualization processes and individual processes of building up one's own biography (Beck, 1992). Life courses are no longer simply given, but (allegedly) dependent on decisions, and if this premise is accepted, the pressure on individuals rises to make the right decisions. Against this background, the essay aims to determine the different action and interpretation patterns with which biographical certainty is created under the conditions of a systematically uncertain world. A typology of biographical certainty developed on the basis of qualitative interviews will be presented. At the end of the essay, results will be integrated in the discourse on a fundamental change or structural rupture within modernity. Finally, the results are examined regarding their significance for health research and practice. What can be considered as the central result is the fact that apparently obsolete certainty strategies, no less than apparently new ones, are effective means of creating biographical certainty and action potential today. Therefore, at a methodological level, the error of equating what is attributed to epochs with specific individual action patterns must be avoided. Rather, when interpreting and classifying results, it must be made explicit where one stands as a scientific observer and what normative implications are involved for the interpretation and classification of results.</dc:description><dc:language>en</dc:language><dc:source>0199285969</dc:source><dc:subject>H</dc:subject><dc:subject>HM</dc:subject><dc:title>Health, Risk and Uncertainty in the Life Course: A Typology of Biographical Certainty Constructions</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Zinn, Jens O.</rioxxterms:author><rioxxterms:contributor>Taylor-Gooby, Peter</rioxxterms:contributor><rioxxterms:contributor>Zinn, Jens O</rioxxterms:contributor><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1057/palgrave.sth.8700033</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:355
Date: 2015-04-28

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
rioxxterms:authorMinimum of 1 value(s) required for rioxxterms:author - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
rioxxterms:authorMinimum of 1 value(s) required for rioxxterms:author - found 0 values
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:355</identifier>
      <datestamp>2015-04-28T13:31:36Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48:4856</setSpec>
      <setSpec>74797065733D6564626F6F6B</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F35</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:language>en</dc:language><dc:publisher>Oxford University Press</dc:publisher><dc:source>9780199285969</dc:source><dc:subject>HV</dc:subject><dc:title>Risk in Social Science</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:contributor>Taylor-Gooby, Peter</rioxxterms:contributor><rioxxterms:contributor>Zinn, Jens O.</rioxxterms:contributor><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2006-10-05</rioxxterms:publication_date><rioxxterms:type>other</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:347
Date: 2017-07-15

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:347</identifier>
      <datestamp>2017-07-15T17:40:51Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48:4831</setSpec>
      <setSpec>7375626A656374733D48:484D</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F34</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The embeddedness of ethnic minority business activity is widely accepted, as researchers increasingly eschew ethno-culturalist explanations of the phenomenon. However, despite the importance of urban processes, studies of ethnic minority enterprise are often ‘spaceless’. This article uses Rekers and van Kempen’s urban spatial framework to assess the experiences of South Asian owners in Birmingham’s restaurant industry. One taken-for-granted but evidently important element to which this article draws attention is location, which emerges as one of the key points of differentiation within the ethnic business community. Location is seen to influence individual access to market potential, an unevenly distributed resource largely shaped by local social geography. At the same time, entry into more lucrative market niches is heavily dependent on the possession of other resources such as capital, information and in some instances management skills.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/347/1/Ethnic_Minority_Enterprise_in_its_Urban_Context_-_IJURR.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Blackwell Publishing</dc:publisher><dc:source>0309-1317 </dc:source><dc:subject>H1</dc:subject><dc:subject>HM</dc:subject><dc:title>Ethnic Minority Enterprise in its Urban Context: South Asian Restaurants in Birmingham</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Ram, Monder</rioxxterms:author><rioxxterms:author>Jones, Trevor</rioxxterms:author><rioxxterms:author>Abbas, Tahir</rioxxterms:author><rioxxterms:author>Sanghera, Balihar</rioxxterms:author><rioxxterms:contributor>MacLachlan, I.</rioxxterms:contributor><rioxxterms:contributor>Syrontinsky, M.</rioxxterms:contributor><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2002-12-12</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1111/1468-2427.00361</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:346
Date: 2017-01-15

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
rioxxterms:version_of_record'http://dx.doi.org/10.1177/09500170122118995 ' is not a valid HTTP URI in rioxxterms:version_of_record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
rioxxterms:version_of_record'http://dx.doi.org/10.1177/09500170122118995 ' is not a valid HTTP URI in rioxxterms:version_of_record
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:346</identifier>
      <datestamp>2017-01-15T13:28:38Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48:4831</setSpec>
      <setSpec>7375626A656374733D48:484D</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F34</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Ethnic minority business activity has often been presented as a vehicle for `upward mobility' for owners and workers alike. Much attention has focused upon the owners themselves. The co-ethnic labour that such employers usually rely upon has often been treated as unproblematic. This paper aims to illuminate the experiences of workers in ethnic minority owned restaurants. In particular, the widely held view that working in a co-ethnic firm serves as an `apprenticeship' for eventual self-employment is explored. &#13;
&#13;
Rather than co-ethnic ties, workers' labour market experiences highlight the importance of the `opportunity structure' in shaping employment choices. The evidence of the current research suggests that the goal of self-employment was not widely held; and although many workers did move around to acquire better paid work, this was not part of a strategic route to becoming a restaurateur. Some workers did cherish such ambitions, but were inhibited by major obstacles. These included intense competition, high start-up costs, and a lack of `know-how'. The labour market and social context of the firm often militated against the hazardous proposition of self-employment. &#13;
&#13;
&#13;
</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/346/1/Apprentice_Entrepreneurs_-_WES.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Sage</dc:publisher><dc:source>0950-0170</dc:source><dc:subject>H1</dc:subject><dc:subject>HM</dc:subject><dc:title>'Apprentice Entrepreneurs’? Ethnic Minority Workers in the Independent Restaurant Sector</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Ram, Monder</rioxxterms:author><rioxxterms:author>Abbas, Tahir</rioxxterms:author><rioxxterms:author>Sanghera, Balihar</rioxxterms:author><rioxxterms:author>Barlow, Gerald</rioxxterms:author><rioxxterms:author>Jones, Trevor</rioxxterms:author><rioxxterms:contributor>MacLachlan, I.</rioxxterms:contributor><rioxxterms:contributor>Syrontinsky, M.</rioxxterms:contributor><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1177/09500170122118995 </rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:345
Date: 2017-01-16

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:345</identifier>
      <datestamp>2017-01-16T15:23:01Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48:4831</setSpec>
      <setSpec>7375626A656374733D48:484D</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F34</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><ali:free_to_read/><dc:description>The ‘family’ is frequently mentioned in assessments of the apparent distinctiveness&#13;
of ethnic minority enterprise. Family involvement can account for the ‘success’ of some ethnic&#13;
groups, and low rates of small business activity in others. Implicitly, such debates are recognition&#13;
of the importance of the nature of the household and small business. However, few studies in&#13;
Britain make explicit the link between household dynamics and ethnic minority business&#13;
activity. This paper examines how households from a variety of ethnic communities impinge&#13;
upon ‘family’ enterprise operating in the independent restaurant sector. In-depth interviews with&#13;
family members from 37 micro-business households are drawn upon to illuminate three&#13;
particular issues: the role of family members in the business; the impact of household dynamics&#13;
on business activity; and the nature of ‘second-generation’ involvement in the family business.&#13;
The � ndings highlight the gendered nature of roles within the micro-business household, and the&#13;
importance of extended family ties to small business activity. However, the involvement of&#13;
predominantly second-generation family members could not be regarded as an example of&#13;
uncomplicated family collectivities at work. Rather, their presence was more a product of limited&#13;
labour market choices, socialisation, and power relations within the household.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/345/1/Making_the_Link_-_CWF.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Taylor &amp; Francis (Routledge)</dc:publisher><dc:source>1366-8803</dc:source><dc:subject>H1</dc:subject><dc:subject>HM</dc:subject><dc:title>Making the link: households and small business activity in a multi-ethnic context</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Ram, Monder</rioxxterms:author><rioxxterms:author>Abbas, Tahir</rioxxterms:author><rioxxterms:author>Sanghera, Balihar</rioxxterms:author><rioxxterms:author>Barlow, Gerald</rioxxterms:author><rioxxterms:author>Jones, Trevor</rioxxterms:author><rioxxterms:contributor>MacLachlan, I.</rioxxterms:contributor><rioxxterms:contributor>Syrontinsky, M.</rioxxterms:contributor><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1080/01405110120089378</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:335
Date: 2015-11-06

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:335</identifier>
      <datestamp>2015-11-06T17:02:36Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48:4831</setSpec>
      <setSpec>7375626A656374733D48:484D</setSpec>
      <setSpec>74797065733D626F6F6B5F73656374696F6E</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F34</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:language>en</dc:language><dc:publisher>Arnold/Oxford University Press</dc:publisher><dc:source>1479-7585</dc:source><dc:subject>H1</dc:subject><dc:subject>HM</dc:subject><dc:title>Stitching the Web into Global Capitalism: two stories</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Miller, Vincent</rioxxterms:author><rioxxterms:contributor>Gauntlett, David</rioxxterms:contributor><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004-01</rioxxterms:publication_date><rioxxterms:type>Book chapter</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:333
Date: 2015-11-09

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:333</identifier>
      <datestamp>2015-11-09T11:23:38Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48</setSpec>
      <setSpec>7375626A656374733D48:484E</setSpec>
      <setSpec>7375626A656374733D48:484D</setSpec>
      <setSpec>74797065733D626F6F6B</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F34</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:language>en</dc:language><dc:publisher>Palgrave Macmillan</dc:publisher><dc:source>9781403912879</dc:source><dc:subject>H</dc:subject><dc:subject>HN</dc:subject><dc:subject>HM</dc:subject><dc:title>Housing decisions in Later Life</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Clough, Roger</rioxxterms:author><rioxxterms:author>Leamy, Mary</rioxxterms:author><rioxxterms:author>Miller, Vincent</rioxxterms:author><rioxxterms:author>Bright, Les</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Book</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:306
Date: 2016-11-22

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:306</identifier>
      <datestamp>2016-11-22T11:51:09Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48</setSpec>
      <setSpec>7375626A656374733D48:484E</setSpec>
      <setSpec>7375626A656374733D48:4831</setSpec>
      <setSpec>7375626A656374733D48:4856</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F33</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>From a socio-cultural standpoint, the concept of otherness is central to explaining the way risk is associated with certain social groups rather than others. In the mental health field, writers have frequently employed the concept of otherness to describe the ways in which mental health service users have been perceived, especially since the implementation of community care policies in the UK in the 1990s. Despite its popularity, few empirical studies have explored the concept of otherness and its use in explaining the risks associated with being mentally ill in any depth, and fewer still have done so in relation to professional practice. The present paper draws upon data from semi-structured interviews with 39 mental health social workers in order to explore the relevance of this concept in risk assessment. The paper employs the concept of liminal otherness as an analytic tool to explain how social workers assessed the risk of clients who were 'difficult to place' because of uncertainty about whether their behaviour was the result of their personality or their mental illness. It is concluded that liminality is relevant to understanding the following: the way in which some service users are found 'in-between' places in terms of service provision; the allocation of responsibility for the management of the risks they represent to social work as a liminal profession; and the symbolic importance of 'the street' as a liminal space within which forms of 'racial otherness' have become central to contemporary constructions of community care.</dc:description><dc:language>en</dc:language><dc:publisher>Routledge Journals</dc:publisher><dc:source>1369-8575</dc:source><dc:subject>H</dc:subject><dc:subject>HN</dc:subject><dc:subject>H1</dc:subject><dc:subject>HV</dc:subject><dc:title>Risk and liminality in mental health social work</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Warner, Joanne</rioxxterms:author><rioxxterms:author>Gabe, Jonathan</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1080/13698570412331323261</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:261
Date: 2016-02-04

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:261</identifier>
      <datestamp>2016-02-04T14:19:23Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D48:484E</setSpec>
      <setSpec>7375626A656374733D48:4831</setSpec>
      <setSpec>7375626A656374733D48:4856</setSpec>
      <setSpec>7375626A656374733D48:484D</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F32</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031:31333730312F34</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133373031</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The rational choice theory of crime and its cognate field of study, situational crime prevention, have exerted a considerable influence in criminal justice policy and criminology. This article argues that, while undeniably useful as a means of reducing property or acquisitive crime, rational choice-inspired situational crime prevention initiatives are limited when it comes to offering protection against a growing number of so-called ‘expressive crimes’. Developing this critique, the article will criticize the sociologically hollow narrative associated with rational choice theories of crime by drawing on recent research in social theory and consumer studies. It argues that the growing tendency among many young individuals to engage in certain forms of criminal decision-making ‘strategies’ may simply be the by-product of a series of subjectivities and emotions that reflect the material values and cultural logic associated with late modern consumerism.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/261/1/hayward2007spol_550.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Blackwell Publishing Ltd</dc:publisher><dc:source>0144-5596</dc:source><dc:subject>HN</dc:subject><dc:subject>H1</dc:subject><dc:subject>HV</dc:subject><dc:subject>HM</dc:subject><dc:title>Situational crime prevention and its discontents: rational choice theory versus the 'culture of now'</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Hayward, Keith J.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2007-06</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1111/j.1467-9515.2007.00550.x</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:248
Date: 2017-09-08

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:248</identifier>
      <datestamp>2017-09-08T08:38:17Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D4B:4B31</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133343030</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Reproductive technologies offer the potential to break down parenthood into a number of constituent parts. These disruptive possibilities mean that the regulation of reproductive technologies holds important potential for study, providing a significant resource that has been little analysed with regard to fatherhood. This study attempts to remedy that lacuna through consideration of a range of recent developments in this area of English law. It reaches two general conclusions. First, while the law regulating reproductive technologies attributes great importance to fatherhood, this is rooted primarily (though not exclusively) in concerns for the symbolic importance of fathers, rather than in more practical considerations such as ensuring financial provision or a second hands-on carer for a child. Secondly, the Human Fertilisation and Embryology Act (1990) contains a clear attempt to protect and entrench the role of the father as completing the nuclear family. However, recent developments suggest that this legal preference for the nuclear family is subject to clear emerging cracks.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/248/1/fatherhood_and_nrts_uncorrected_proofs.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Wiley</dc:publisher><dc:source>0026-7961</dc:source><dc:subject>K1</dc:subject><dc:title>Fragmenting Fatherhood: The Regulation of Reproductive Technologies</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Sheldon, Sally</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2005</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1111/j.1468-2230.2005.00550.x</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:244
Date: 2016-02-05

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:244</identifier>
      <datestamp>2016-02-05T10:26:23Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D4B</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133343030</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><ali:free_to_read/><dc:description>Against the historical backdrop of the codification debate in nineteenth century Germany, this article traces the reassertion of ‘legal science’ as an autonomous source of European legal integration in current legal and political discourse about the harmonization of European private law. The article argues that a grasp of widely shared ideas about the role and function of legal science and legal scientists is vital both towards an understanding of the extraordinary impact of the academic project of a European civil code on legal and political discourse in the Union in particular and towards furthering the theory of legal fields in general.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/244/1/European_Brotherhood_26OCT07DP.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Blackwell Publishing</dc:publisher><dc:source>0897-6546</dc:source><dc:subject>K</dc:subject><dc:title>The European Brotherhood of Lawyers: The Reinvention of Legal Science in European Private Law</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Schepel, Harm</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2007-03</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:218
Date: 2016-03-10

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
dc:identifierMinimum of 1 value(s) required for dc:identifier - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:218</identifier>
      <datestamp>2016-03-10T15:39:48Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D4B</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133343030</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>While gender equality has been a matter of some concern for EU law and policy makers over the past half century, this concern has tended, at least historically, to focus upon equal treatment in employment and has not yet materialised into the delivery of a broader package of civil, political, and social rights for women. Taking the concept of EU citizenship as a framework within which to view the promotion of gender equality, this article assesses the debate on the constitutional future of the EU. This is with a view to examining the possible amelioration of women's social position through the exploitation of opportunities that the constitutionalisation of EU law presents. Looking at women's citizenship through the lens of political rights to participate in the debate on the EU's future, together with examining substantive aspects of the Constitutional Treaty for their gender equality content, the article suggests that a more comprehensive endeavour by all institutional actors to engage in gender mainstreaming is needed in order to give effect to a broader form of equality between women and men.</dc:description><dc:language>en</dc:language><dc:publisher>Wiley</dc:publisher><dc:relation>http://www.europeanlawjournal.com</dc:relation><dc:source>1351-5993</dc:source><dc:subject>K</dc:subject><dc:title>Gender Equality, Citizenship, and the EU's Constitutional Future</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Millns, Susan</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1111/j.1468-0386.2007.00365.x</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:205
Date: 2017-01-17

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
rioxxterms:version_of_record'DOI not available' is not a valid HTTP URI in rioxxterms:version_of_record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
rioxxterms:version_of_record'DOI not available' is not a valid HTTP URI in rioxxterms:version_of_record
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:205</identifier>
      <datestamp>2017-01-17T03:02:19Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D47:4745</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133313030:31333130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/205/1/Harrop-_indigenous_peoples_and_conservation.pdf</dc:identifier><dc:language>en</dc:language><dc:source>0025-1283</dc:source><dc:subject>GE</dc:subject><dc:title>Human diversity and the diversity of life: international regulation of the role of indigenous and rural human communities in conservation</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Harrop, Stuart R.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2003</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>DOI not available</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:200
Date: 2015-05-11

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:200</identifier>
      <datestamp>2015-05-11T13:50:28Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D47:4745</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133313030:31333130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The Po'ouli (Melamprosops phaeosoma), a Hawaiian honeycreeper endemic to Maui, has a population of only three known individuals; no breeding pair currently exists, and their home ranges are too far apart for breeding to occur. Without timely intervention this monotypic genus will likely go extinct. Conservationists have faced a dilemma: facilitate breeding amongst the known individuals, manage their ecosystem to benefit uncounted Po'ouli, or a combination of both? Po'ouli biology is poorly known - but their remote home ranges are closely monitored. A State and Federal Environmental Assessment in 1999 recommended that one Po'ouli be translocated into the home range of another in an attempt to facilitate breeding. This first manipulative recovery action was achieved in April 2002, and provided valuable new information for future captive management efforts, but upon release, radio telemetry confirmed that the translocated bird returned to its own home range after one day. We describe the recent progress that has been made to recover the Po'ouli, and critically evaluate the Po'ouli case study and the lessons learned from it that can help expedite recovery of other birds of extreme rarity. (C) 2003 Elsevier Ltd. All rights reserved.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/200/1/Groombridge_%26_Massey_%26_VanderWerf_Po%27ouliByTranslocation_July_2004.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Elsevier Science B.V., Amsterdam.</dc:publisher><dc:source>0006-3207</dc:source><dc:subject>GE</dc:subject><dc:title>An attempt to recover the Po'ouli by translocation and an appraisal of recovery strategy for bird species of extreme rarity</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Groombridge, Jim J.</rioxxterms:author><rioxxterms:author>Massey, J. Gregory</rioxxterms:author><rioxxterms:author>Bruch, James C.</rioxxterms:author><rioxxterms:author>Malcolm, Trent R.</rioxxterms:author><rioxxterms:author>Brosius, Christopher N.</rioxxterms:author><rioxxterms:author>Okada, Marcy M.</rioxxterms:author><rioxxterms:author>Sparklin, Bill D.</rioxxterms:author><rioxxterms:author>Fretz, J. Scott</rioxxterms:author><rioxxterms:author>VanderWerf, Eric A.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004-07</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1016/j.biocon.2003.06.005</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:163
Date: 2017-06-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
rioxxterms:apc'not_required' is not a valid term from the RIOXX APC vocabulary in rioxxterms:apc
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:163</identifier>
      <datestamp>2017-06-27T13:24:19Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D47:474E</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133313030</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/163/1/Johns-TeenagePregnancy-244-1.pdf</dc:identifier><dc:language>en</dc:language><dc:source>0959-8138</dc:source><dc:subject>GN</dc:subject><dc:title>Teenage pregnancy is not a public health problem</dc:title><rioxxterms:apc>not_required</rioxxterms:apc><rioxxterms:author>Lawlor, Debbie A</rioxxterms:author><rioxxterms:author>Shaw, Mary</rioxxterms:author><rioxxterms:author>Johns, Sarah E.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001-12-15</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1136/bmj.323.7326.1428</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:153
Date: 2017-01-17

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:153</identifier>
      <datestamp>2017-01-17T15:21:17Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D47:474E</setSpec>
      <setSpec>74797065733D626F6F6B5F73656374696F6E</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133313030</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><ali:free_to_read/><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/153/1/23129-1.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Routledge</dc:publisher><dc:source>0 415 22905 7</dc:source><dc:subject>GN</dc:subject><dc:title>The Violence in Identity</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Bowman, Glenn W.</rioxxterms:author><rioxxterms:contributor>Schmidt, Bettina</rioxxterms:contributor><rioxxterms:contributor>Schroeder, Ingo</rioxxterms:contributor><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Book chapter</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:151
Date: 2017-07-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:151</identifier>
      <datestamp>2017-07-27T13:16:06Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D47:474E</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133313030</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><ali:free_to_read/><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/151/1/Bowman.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Wiley</dc:publisher><dc:source>0964-0282</dc:source><dc:subject>GN</dc:subject><dc:title>Constitutive Violence and the Nationalist Imaginary: Antagonism and Defensive Solidarity in 'Palestine' and 'Former Yugoslavia'</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Bowman, Glenn W.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2003-10</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1111/j.1469-8676.2003.tb00081.x</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:133
Date: 2016-04-28

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:133</identifier>
      <datestamp>2016-04-28T11:28:34Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D4B:4B31</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133343030</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><ali:free_to_read/><dc:description>This article focuses upon the writings of John R. Bolton who was for four years US Under-Secretary of State for Arms Control and International Security. He is currently the US Ambassador to the United Nations. His position with regard to international law is, at least for non-Americans, extraordinary, but also extraordinarily important since it resonates with the views of many in the current Bush administration. In essence, he is sceptical of the entire category of international law and argues that it cannot ever be accepted as superior to US domestic law. He doubts that it can be distinguished from international relations. These views need to be taken seriously if the implications for the world of diplomacy and international relations, and indeed domestic law, are to be understood. This the article attempts to do. </dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/133/1/JOhn_Bolton_and_the_United_states_retreat_from_international_law.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Sage</dc:publisher><dc:source>0964-6639</dc:source><dc:subject>K1</dc:subject><dc:title>John Bolton and the US Retreat from International Law</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Haslam, Emily</rioxxterms:author><rioxxterms:author>Mansell, Wade</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2005</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1177/0964663905057591</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:113
Date: 2016-04-28

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:113</identifier>
      <datestamp>2016-04-28T11:26:23Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D4B:4B31</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3133303030:3133343030</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><ali:free_to_read/><dc:description>Hostels and other forms of housing where support services are provided as an intrinsic part of the accommodation package have traditionally been developed by the voluntary sector at a distance from conditional state welfare. Supporting People is an innovative and ambitious programme which in effect annexes supported housing and, in return for a commitment to improved provision, promises certainty of income and professional prestige. Supporting People provides an example of contemporary social policy. It attempts to address both the failures of the ‘old’ welfare state and the anxieties of the neo-liberal state. It does this through a distinct ‘third way’ form of regulation which extends new public management practices into a new regulatory arena and places a particular emphasis on ‘joined-up’ thinking, risk management and the ideological pragmatism of ‘what works’. This has particular consequences for the diverse range of both providers and residents who are disciplined through a variety of mechanisms to deliver social progress for the state</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/113/1/Someone_to_watch_over_me.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Sage</dc:publisher><dc:source>0964-6639</dc:source><dc:subject>K1</dc:subject><dc:title>Someone to Watch over Me: Making Supported Housing Work</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Carr, Helen</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2005</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1177/0964663905054910</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:106
Date: 2017-06-29

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:106</identifier>
      <datestamp>2017-06-29T04:47:47Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51:5148:5148343236</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>We present here a draft genome sequence of the red jungle fowl, Gallus gallus. Because the chicken is a modern descendant of the dinosaurs and the first non-mammalian amniote to have its genome sequenced, the draft sequence of its genome--composed of approximately one billion base pairs of sequence and an estimated 20,000-23,000 genes--provides a new perspective on vertebrate genome evolution, while also improving the annotation of mammalian genomes. For example, the evolutionary distance between chicken and human provides high specificity in detecting functional elements, both non-coding and coding. Notably, many conserved non-coding sequences are far from genes and cannot be assigned to defined functional classes. In coding regions the evolutionary dynamics of protein domains and orthologous groups illustrate processes that distinguish the lineages leading to birds and mammals. The distinctive properties of avian microchromosomes, together with the inferred patterns of conserved synteny, provide additional insights into vertebrate chromosome architecture.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/106/1/Hillier_et_al_2004%2C_Nature_432%2C_695-716.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Nature Publishing Group</dc:publisher><dc:relation>http://www.ncbi.nlm.nih.gov/pubmed/15592404</dc:relation><dc:relation>https://www.researchgate.net/publication/232763703_Sequence_and_comparative_analysis_of_the_chicken_genome_provide_unique_perspectives_on_vertebrate_evolution</dc:relation><dc:relation>http://www.worldcat.org/title/sequence-and-comparative-analysis-of-the-chicken-genome-provide-unique-perspectives-on-vertebrate-evolution/oclc/98659165&amp;referer=brief_results</dc:relation><dc:relation>http://www.worldcat.org/title/sequence-and-comparative-analysis-of-the-chicken-genome-provide-unique-perspectives-on-vertebrate-evolution/oclc/4654201874&amp;referer=brief_results</dc:relation><dc:relation>http://www.worldcat.org/title/sequence-and-comparative-analysis-of-the-chicken-genome-provide-unique-perspectives-on-vertebrate-evolution/oclc/757559811&amp;referer=brief_results</dc:relation><dc:relation>http://www.worldcat.org/title/sequence-and-comparative-analysis-of-the-chicken-genome-provide-unique-perspectives-on-vertebrate-evolution/oclc/899125727&amp;referer=brief_results</dc:relation><dc:relation>http://www.worldcat.org/title/sequence-and-comparative-analysis-of-the-chicken-genome-provide-unique-perspectives-on-vertebrate-evolution/oclc/842693641&amp;referer=brief_results</dc:relation><dc:relation>http://www.worldcat.org/title/sequence-and-comparative-analysis-of-the-chicken-genome-provide-unique-perspectives-on-vertebrate-evolution/oclc/111652055&amp;referer=brief_results</dc:relation><dc:relation>http://www.worldcat.org/title/sequence-and-comparative-analysis-of-the-chicken-genome-provide-unique-perspectives-on-vertebrate-evolution/oclc/202397944&amp;referer=brief_results</dc:relation><dc:relation>http://www.worldcat.org/title/sequence-and-comparative-analysis-of-the-chicken-genome-provide-unique-perspectives-on-vertebrate-evolution/oclc/892499779&amp;referer=brief_results</dc:relation><dc:relation>http://eurekamag.com/research/004/315/004315226.php</dc:relation><dc:relation>https://getinfo.de/app/Sequence-and-comparative-analysis-of-the-chicken/id/npg%3Adoi~10.1038%252Fnature03154</dc:relation><dc:relation>https://getinfo.de/app/Sequence-and-comparative-analysis-of-the-chicken/id/BLSE%3ARN160233835</dc:relation><dc:source>0028-0836</dc:source><dc:subject>QH426</dc:subject><dc:title>Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Hillier, LaDeana W</rioxxterms:author><rioxxterms:author>Miller, Webb</rioxxterms:author><rioxxterms:author>Birney, Ewan</rioxxterms:author><rioxxterms:author>Warren, Wesley</rioxxterms:author><rioxxterms:author>Hardison, Ross C</rioxxterms:author><rioxxterms:author>Ponting, Chris P</rioxxterms:author><rioxxterms:author>Bork, Peer</rioxxterms:author><rioxxterms:author>Burt, Peer</rioxxterms:author><rioxxterms:author>Groenen, Martien A M</rioxxterms:author><rioxxterms:author>Delany, Mary E</rioxxterms:author><rioxxterms:author>Dodgson, Jerry B</rioxxterms:author><rioxxterms:author>Chinwalla, Asif</rioxxterms:author><rioxxterms:author>Cliften, Paul F</rioxxterms:author><rioxxterms:author>Clifton, Sandra W</rioxxterms:author><rioxxterms:author>Delehaunty, Kimberly D</rioxxterms:author><rioxxterms:author>Fronick, Catrina</rioxxterms:author><rioxxterms:author>Fulton, Robert S</rioxxterms:author><rioxxterms:author>Graves, Tina A</rioxxterms:author><rioxxterms:author>Kremitzki, Colin</rioxxterms:author><rioxxterms:author>Layman, Dan</rioxxterms:author><rioxxterms:author>Magrini, Vincent</rioxxterms:author><rioxxterms:author>McPherson, John D</rioxxterms:author><rioxxterms:author>Miner, Tracie L</rioxxterms:author><rioxxterms:author>Minx, Patrick</rioxxterms:author><rioxxterms:author>Nash, William E</rioxxterms:author><rioxxterms:author>Nhan, Michael N</rioxxterms:author><rioxxterms:author>Nelson, Joanne O</rioxxterms:author><rioxxterms:author>Oddy, Lachlan G</rioxxterms:author><rioxxterms:author>Pohl, Craig S</rioxxterms:author><rioxxterms:author>Randall-Maher, Jennifer</rioxxterms:author><rioxxterms:author>Smith, Scott M</rioxxterms:author><rioxxterms:author>Wallis, John W</rioxxterms:author><rioxxterms:author>Yang, Shiaw-Pyng</rioxxterms:author><rioxxterms:author id="http://orcid.org/0000-0003-3584-4644">Romanov, Michael N</rioxxterms:author><rioxxterms:author>Rondelli, Catherine M</rioxxterms:author><rioxxterms:author>Paton, Bob</rioxxterms:author><rioxxterms:author>Smith, Jacqueline</rioxxterms:author><rioxxterms:author>Morrice, David</rioxxterms:author><rioxxterms:author>Daniels, Laura</rioxxterms:author><rioxxterms:author>Tempest, Helen G</rioxxterms:author><rioxxterms:author>Robertson, Lindsay</rioxxterms:author><rioxxterms:author>Masabanda, Julio S</rioxxterms:author><rioxxterms:author>Griffin, Darren K</rioxxterms:author><rioxxterms:author>Vignal, Alain</rioxxterms:author><rioxxterms:author>Fillon, Valerie</rioxxterms:author><rioxxterms:author>Jacobbson, Lina</rioxxterms:author><rioxxterms:author>Kerje, Susanne</rioxxterms:author><rioxxterms:author>Andersson, Leif</rioxxterms:author><rioxxterms:author>Crooijmans, Richard P M A</rioxxterms:author><rioxxterms:author>Aerts, Jan</rioxxterms:author><rioxxterms:author>van der Poel, Jan J</rioxxterms:author><rioxxterms:author>Ellegren, Hans</rioxxterms:author><rioxxterms:author>Caldwell, Randolph B</rioxxterms:author><rioxxterms:author>Hubbard, Simon J</rioxxterms:author><rioxxterms:author>Grafham, Darren V</rioxxterms:author><rioxxterms:author>Kierzek, Andrzej M</rioxxterms:author><rioxxterms:author>McLaren, Stuart R</rioxxterms:author><rioxxterms:author>Overton, Ian M</rioxxterms:author><rioxxterms:author>Arakawa, Hiroshi</rioxxterms:author><rioxxterms:author>Beattie, Kevin J</rioxxterms:author><rioxxterms:author>Bezzubov, Yuri</rioxxterms:author><rioxxterms:author>Boardman, Paul E</rioxxterms:author><rioxxterms:author>Bonfield, James K</rioxxterms:author><rioxxterms:author>Croning, Michael D R</rioxxterms:author><rioxxterms:author>Davies, Robert M</rioxxterms:author><rioxxterms:author>Francis, Matthew D</rioxxterms:author><rioxxterms:author>Humphray, Sean J</rioxxterms:author><rioxxterms:author>Scott, Carol E</rioxxterms:author><rioxxterms:author>Taylor, Ruth G</rioxxterms:author><rioxxterms:author>Tickle, Cheryll</rioxxterms:author><rioxxterms:author>Brown, William R A</rioxxterms:author><rioxxterms:author>Rogers, Jane</rioxxterms:author><rioxxterms:author>Buerstedde, Jean-Marie</rioxxterms:author><rioxxterms:author>Wilson, Stuart A</rioxxterms:author><rioxxterms:author>Stubbs, Lisa</rioxxterms:author><rioxxterms:author>Ovcharenko, Ivan</rioxxterms:author><rioxxterms:author>Gordon, Laurie</rioxxterms:author><rioxxterms:author>Lucas, Susan</rioxxterms:author><rioxxterms:author>Miller, Marcia M</rioxxterms:author><rioxxterms:author>Inoko, Hidetoshi</rioxxterms:author><rioxxterms:author>Shiina, Takashi</rioxxterms:author><rioxxterms:author>Kaufman, Jim</rioxxterms:author><rioxxterms:author>Salomonsen, Jan</rioxxterms:author><rioxxterms:author>Skjoedt, Karsten</rioxxterms:author><rioxxterms:author>Wong, Gane Ka-Shu</rioxxterms:author><rioxxterms:author>Wang, Jun</rioxxterms:author><rioxxterms:author>Liu, Bin</rioxxterms:author><rioxxterms:author>Wang, Jian</rioxxterms:author><rioxxterms:author>Yu, Jun</rioxxterms:author><rioxxterms:author>Yang, Huanming</rioxxterms:author><rioxxterms:author>Nefedov, Mikhail</rioxxterms:author><rioxxterms:author>Koriabine, Maxim</rioxxterms:author><rioxxterms:author>de Jong, Pieter J</rioxxterms:author><rioxxterms:author>Goodstadt, Leo</rioxxterms:author><rioxxterms:author>Webber, Caleb</rioxxterms:author><rioxxterms:author>Dickens, Nicholas J</rioxxterms:author><rioxxterms:author>Letunic, Ivica</rioxxterms:author><rioxxterms:author>Suyama, Mikita</rioxxterms:author><rioxxterms:author>Torrents, David</rioxxterms:author><rioxxterms:author>von Mering, Christian</rioxxterms:author><rioxxterms:author>Zdobnov, Evgeny M</rioxxterms:author><rioxxterms:author>Makova, Kateryna</rioxxterms:author><rioxxterms:author>Nekrutenko, Anton</rioxxterms:author><rioxxterms:author>Elnitski, Laura</rioxxterms:author><rioxxterms:author>Eswara, Pallavi</rioxxterms:author><rioxxterms:author>King, David C</rioxxterms:author><rioxxterms:author>Yang, Shan</rioxxterms:author><rioxxterms:author>Tyekucheva, Svitlana</rioxxterms:author><rioxxterms:author>Radakrishnan, Anusha</rioxxterms:author><rioxxterms:author>Harris, Robert S</rioxxterms:author><rioxxterms:author>Chiaromonte, Francesca</rioxxterms:author><rioxxterms:author>Taylor, James</rioxxterms:author><rioxxterms:author>He, Jianbin</rioxxterms:author><rioxxterms:author>Rijnkels, Monique</rioxxterms:author><rioxxterms:author>Griffiths-Jones, Sam</rioxxterms:author><rioxxterms:author>Ureta-Vidal, Abe</rioxxterms:author><rioxxterms:author>Hoffman, Michael M</rioxxterms:author><rioxxterms:author>Severin, Jessica</rioxxterms:author><rioxxterms:author>Searle, Stephen M J</rioxxterms:author><rioxxterms:author>Law, Andy S</rioxxterms:author><rioxxterms:author>Speed, David</rioxxterms:author><rioxxterms:author>Waddington, David</rioxxterms:author><rioxxterms:author>Cheng, Ze</rioxxterms:author><rioxxterms:author>Tuzun, Eray</rioxxterms:author><rioxxterms:author>Eichler, Evan</rioxxterms:author><rioxxterms:author>Bao, Zhirong</rioxxterms:author><rioxxterms:author>Flicek, Paul</rioxxterms:author><rioxxterms:author>Shteynberg, David D</rioxxterms:author><rioxxterms:author>Brent, Michael R</rioxxterms:author><rioxxterms:author>Bye, Jacqueline M</rioxxterms:author><rioxxterms:author>Huckle, Elizabeth J</rioxxterms:author><rioxxterms:author>Chatterjee, Sourav</rioxxterms:author><rioxxterms:author>Dewey, Colin</rioxxterms:author><rioxxterms:author>Pachter, Lior</rioxxterms:author><rioxxterms:author>Kouranov, Andrei</rioxxterms:author><rioxxterms:author>Mourelatos, Zissimos</rioxxterms:author><rioxxterms:author>Hatzigeorgiou, Artemis G</rioxxterms:author><rioxxterms:author>Paterson, Andrew H</rioxxterms:author><rioxxterms:author>Ivarie, Robert</rioxxterms:author><rioxxterms:author>Brandstrom, Mikael</rioxxterms:author><rioxxterms:author>Axelsson, Erik</rioxxterms:author><rioxxterms:author>Backström, Niclas</rioxxterms:author><rioxxterms:author>Berlin, Sofia</rioxxterms:author><rioxxterms:author>Webster, Matthew T</rioxxterms:author><rioxxterms:author>Pourquie, Olivier</rioxxterms:author><rioxxterms:author>Reymond, Alexandre</rioxxterms:author><rioxxterms:author>Ucla, Catherine</rioxxterms:author><rioxxterms:author>Antonarakis, Stylianos E</rioxxterms:author><rioxxterms:author>Long, Manyuan</rioxxterms:author><rioxxterms:author>Emerson, J J</rioxxterms:author><rioxxterms:author>Betran, Esther</rioxxterms:author><rioxxterms:author>Dupanloup, Isabelle</rioxxterms:author><rioxxterms:author>Kaessmann, Henrik</rioxxterms:author><rioxxterms:author>Hinrichs, Angie S</rioxxterms:author><rioxxterms:author>Bejerano, Gill</rioxxterms:author><rioxxterms:author>Furey, Terrence S</rioxxterms:author><rioxxterms:author>Harte, Rachel A</rioxxterms:author><rioxxterms:author>Raney, Brian</rioxxterms:author><rioxxterms:author>Siepel, Adam</rioxxterms:author><rioxxterms:author>Kent, W James</rioxxterms:author><rioxxterms:author>Haussler, David</rioxxterms:author><rioxxterms:author>Eyras, Eduardo</rioxxterms:author><rioxxterms:author>Castelo, Robert</rioxxterms:author><rioxxterms:author>Abril, Josep F</rioxxterms:author><rioxxterms:author>Castellano, Sergi</rioxxterms:author><rioxxterms:author>Camara, Francisco</rioxxterms:author><rioxxterms:author>Parra, Genis</rioxxterms:author><rioxxterms:author>Guigo, Roderic</rioxxterms:author><rioxxterms:author>Bourque, Guillaume</rioxxterms:author><rioxxterms:author>Tesler, Glenn</rioxxterms:author><rioxxterms:author>Pevzner, Pavel A.</rioxxterms:author><rioxxterms:author>Smit, Arian F. A.</rioxxterms:author><rioxxterms:author>Fulton, Lucinda A.</rioxxterms:author><rioxxterms:author>Mardis, Elaine R.</rioxxterms:author><rioxxterms:author>Wilson, Richard K.</rioxxterms:author><rioxxterms:author>International Chicken Genome Sequencing Consortium</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004-12-09</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1038/nature03154</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:104
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:104</identifier>
      <datestamp>2016-04-27T15:48:07Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F33</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Chicken genome mapping is important for a range of scientific disciplines. The ability to distinguish chromosomes of the chicken and other birds is thus a priority. Here we describe the molecular cytogenetic characterization of each chicken chromosome using chromosome painting and mapping of individual clones by FISH. Where possible, we have assigned the chromosomes to known linkage groups. We propose, on the basis of size, that the NOR chromosome is approximately the size of chromosome 22; however, we suggest that its original assignment of 16 should be retained. We also suggest a definitive chromosome classification system and propose that the probes developed here will find wide utility in the fields of developmental biology, DT40 studies, agriculture, vertebrate genome organization, and comparative mapping of avian species.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/104/1/Masabanda_et_al_2004%2C_Genetics.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Genetics Society of America</dc:publisher><dc:source>0016-6731</dc:source><dc:subject>Q</dc:subject><dc:title>Molecular cytogenetic definition of the chicken genome: the first complete avian karyotype.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Masabanda, Julio S.</rioxxterms:author><rioxxterms:author>Burt, David W.</rioxxterms:author><rioxxterms:author>O'Brien, Patricia C. M.</rioxxterms:author><rioxxterms:author>Vignal, Alain</rioxxterms:author><rioxxterms:author>Fillon, Valerie</rioxxterms:author><rioxxterms:author>Walsh, Philippa S.</rioxxterms:author><rioxxterms:author>Cox, Helen</rioxxterms:author><rioxxterms:author>Tempest, Helen G.</rioxxterms:author><rioxxterms:author>Smith, Jacqueline</rioxxterms:author><rioxxterms:author>Habermann, Felix A.</rioxxterms:author><rioxxterms:author>Schmid, Michael</rioxxterms:author><rioxxterms:author>Matsuda, Yoichi</rioxxterms:author><rioxxterms:author>Ferguson-Smith, Malcolm A.</rioxxterms:author><rioxxterms:author>Crooijmans, Richard P.M.A.</rioxxterms:author><rioxxterms:author>Groenen, Martien A. M.</rioxxterms:author><rioxxterms:author>Griffin, Darren K.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:102
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:102</identifier>
      <datestamp>2016-04-27T15:42:19Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F33</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>A combination of FISH and RH mapping was used to study the evolution of sex chromosome genes in the pig. In total, 19 genes were identified, including 3 PAR genes (STS, KAL, PRK). The gene order of the porcine X Chromosome (Chr) closely resembled the human X Chr (PRK/STS/KAL-AMELX-EIF2s3X/ZFX-USP9X-DBX-SMCX), suggesting that the porcine X has undergone very little rearrangement during evolution. For the porcine Y Chr, two linkage groups of 10 NRY genes were found, and the following order was established: Ypter-(AMELY-EIF2S3Y/ZFY-USP9Y-DBY/UTY)-(TSPY-SMCY-UBE1Y-SRY)-CEN. This gene order showed greater conservation with the murine Y than with the human Y Chr. In addition, all porcine Y Chr genes mapped to Yp, which is similar to the mouse and included EIF2s3Y and UBE1Y, which are not present in humans. Interestingly, complete conservation of X/Y homologous gene order was found between the pig X and Y Chrs, indicating that the porcine Y Chr has not undergone extensive reorganisation with respect to the X. This suggests that the order of the X/Y homologous genes of the porcine X and Y Chrs may closely resemble the ancestral gene order of the eutherian sex chromosomes.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/102/1/Quilter_et_al_2003%2C_Mammalian_Genome_13.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Springer-Verlag</dc:publisher><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=12420137</dc:relation><dc:source>0938-8990</dc:source><dc:subject>Q</dc:subject><dc:title>A mapping and evolutionary study of porcine sex chromosome genes.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Quilter, Claire R</rioxxterms:author><rioxxterms:author>Blott, Sarah C</rioxxterms:author><rioxxterms:author>Mileham, Alan J</rioxxterms:author><rioxxterms:author>Affara, Nabeel</rioxxterms:author><rioxxterms:author>Sargent, Carole A</rioxxterms:author><rioxxterms:author>Griffin, Darren K.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2002</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1007/s00335-002-3026-1</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:100
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:100</identifier>
      <datestamp>2016-04-27T15:40:10Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>BACKGROUND: The clinical significance of the increased concentrations of cardiac troponins observed in patients with end stage renal disease (ESRD) in the absence of an acute coronary syndrome (ACS) is controversial. One proposed explanation is that immunoreactive fragments of cardiac troponin T (cTnT) accumulate in ESRD. We used gel-filtration chromatography (GFC) to ascertain whether fragments of cTnT, which could cross-react in the commercial diagnostic immunoassay (Roche Diagnostics), were the cause of the increased cTnT in the serum of patients with ESRD. METHODS: We subjected sera from ESRD patients (n = 21) receiving dialysis and having increased cTnT concentrations to size-separation GFC. We detected cTnT in the chromatography fractions by use of the same antibodies used in the commercial assay for serum cTnT. RESULTS: In all patients, cTnT immunoreactivity eluted as a major, homogeneous peak in an identical position between the peaks of serum prolactin [relative molecular mass (Mr) 23,000] and albumin (Mr 67,000): the elution pattern of cTnT in samples obtained from ACS patients was identical to that of the ESRD patients. There was no evidence that low-molecular-mass (Mr &lt; 23,000) cTnT fragments were the cause of the increased cTnT in the patients studied. CONCLUSIONS: The form of cTnT observed in the serum of patients with kidney failure and immunoreactive in the diagnostic assay is predominantly the free intact form, as in patients with ACS. Our data are consistent with the view that circulating cTnT in renal failure reflects cardiac pathology.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/100/4/Abbas_et_al%252C_Clin_Chem_51%252C_2059-2066.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Association for Clinical Chemistry</dc:publisher><dc:source>0009-9147</dc:source><dc:subject>Q</dc:subject><dc:title>Cardiac troponin T circulates in the free, intact form in patients with kidney failure.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Fahie-Wilson, Michael N.</rioxxterms:author><rioxxterms:author>Carmichael, David J.</rioxxterms:author><rioxxterms:author>Delaney, Michael P.</rioxxterms:author><rioxxterms:author>Stevens, Paul E.</rioxxterms:author><rioxxterms:author>Hall, Elizabeth M</rioxxterms:author><rioxxterms:author>Lamb, Edmund J.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2006</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1373/clinchem.2005.062307</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:98
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:98</identifier>
      <datestamp>2016-04-27T15:36:11Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>BACKGROUND: Serum cardiac troponin concentrations are commonly increased in end-stage renal disease (ESRD) in the absence of an acute coronary syndrome (ACS). The data on cardiac troponin I (cTnI) are more variable than those for cardiac troponin T (cTnT). There is little information on cardiac troponin concentrations in patients with chronic kidney disease (CKD) who have not commenced dialysis. METHODS: We studied 222 patients: 56 had stage 3 (moderate CKD); 70 stage 4 (severe CKD); and 96 stage 5 (kidney failure). Patients underwent echocardiography and were followed prospectively for a median of 19 months; all-cause mortality was recorded. RESULTS: Overall, serum cTnT was increased above the 99th percentile reference limit in 43% of all CKD patients studied, compared with 18% for cTnI. Serum cTnT and cTnI concentrations were more commonly increased in the presence of more severe CKD (11 and 6 patients in stage 3, 27 and 8 in stage 4, and 57 and 24 in stage 5 (P &lt; 0.0001 and &lt;0.02, respectively). Among 38 patients with detectable cTnI, 32 had detectable cTnT (r(s) = 0.67; P &lt; 0.0001). There was evidence that decreasing estimated glomerular filtration rate increased the odds of having detectable cTnT (P &lt; 0.001) but not cTnI (P = 0.128). There was no evidence to support an adjusted association of detectable cardiac troponins with increasing left ventricular mass index. Increased cTnT (P = 0.0097), but not cTnI, was associated with decreased survival. CONCLUSIONS: Increased cTnT and cTnI concentrations are relatively common in predialysis CKD patients, in the absence of an ACS, including among those with stage 3 disease. The presence of left ventricular hypertrophy alone does not explain these data. Detectable cTnT was a marker of decreased survival.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/98/4/Fahie-Wilson_et_al_2006%252C_Clin_Chem_52%252C_414-420.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Association for Clinical Chemistry</dc:publisher><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=16166165</dc:relation><dc:source>0009-9147</dc:source><dc:subject>Q</dc:subject><dc:title>Cardiac troponins and renal function in nondialysis patients with chronic kidney disease.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Abbas, Nasir A.</rioxxterms:author><rioxxterms:author>John, R. Ian</rioxxterms:author><rioxxterms:author>Webb, Michelle C.</rioxxterms:author><rioxxterms:author>Kempson, Michelle E.</rioxxterms:author><rioxxterms:author>Potter, Aisling N</rioxxterms:author><rioxxterms:author>Price, Christopher P.</rioxxterms:author><rioxxterms:author>Vickery, Susan</rioxxterms:author><rioxxterms:author>Lamb, Edmund J.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2005</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1373/clinchem.2005.055665</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:97
Date: 2017-06-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:97</identifier>
      <datestamp>2017-06-27T05:29:03Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/97/1/Lamb_et_al_2004%2C_Clin_Chem_50%2C_1848-1851.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Association for Clinical Chemistry</dc:publisher><dc:source>0009-9147</dc:source><dc:subject>Q</dc:subject><dc:title>Diagnostic accuracy of cystatin C as a marker of kidney disease in patients with multiple myeloma: calculated glomerular filtration rate formulas are equally useful.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Lamb, Edmund J.</rioxxterms:author><rioxxterms:author>Stowe, Helen</rioxxterms:author><rioxxterms:author>Simpson, David E.</rioxxterms:author><rioxxterms:author>Coakley, Anthony J.</rioxxterms:author><rioxxterms:author>Newman, David J.</rioxxterms:author><rioxxterms:author>Leahy, Maeve</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:95
Date: 2017-06-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:95</identifier>
      <datestamp>2017-06-27T05:29:00Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/95/1/Stowe_et_al_2001%2C_Clin_Chem_47%2C_1482-1485.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Association for Clinical Chemistry</dc:publisher><dc:source>0009-9147</dc:source><dc:subject>Q</dc:subject><dc:title>Analytical performance of a particle-enhanced nephelometric immunoassay for serum cystatin C using rate analysis.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Stowe, Helen</rioxxterms:author><rioxxterms:author>Lawrence, David</rioxxterms:author><rioxxterms:author>Newman, David J.</rioxxterms:author><rioxxterms:author>Lamb, Edmund J.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:93
Date: 2017-06-29

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:93</identifier>
      <datestamp>2017-06-29T04:46:33Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>An elderly patient with no abnormal bleeding presented with prolongation of the activated partial thromboplastin time (aPTT). Preincubation of plasma with aPTT reagent caused shortening of the abnormal clotting time. Plasma prekallikrein (PK) activity and antigen were &lt;5 u/dL. Molecular analysis showed a homozygous Arg94Stop substitution in the PK gene, predicted to prevent expression of the mutant allele. The five heterozygous offspring of the proband each showed a normal aPTT but reduced PK activity and antigen. This is the first description of a kindred in which absence of expression of one or both PK alleles has been confirmed by genotype.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/93/1/Jones_et_al_2004%2C_British_J_of_Haematology_217%2C_220-223.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>BLACKWELL PUBLISHING LTD, 9600 GARSINGTON RD, OXFORD OX4 2DG, OXON, ENGLAND</dc:publisher><dc:source>0007-1048</dc:source><dc:subject>Q</dc:subject><dc:title>Severe prekallikrein deficiency associated with homozygosity for an Arg94Stop nonsense mutation</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Jones, David Wynne</rioxxterms:author><rioxxterms:author>Russell, Geoffrey</rioxxterms:author><rioxxterms:author>Allford, Sarah L.</rioxxterms:author><rioxxterms:author>Burdon, Kathryn</rioxxterms:author><rioxxterms:author>Hawkins, Gregory A.</rioxxterms:author><rioxxterms:author>Bowden, Donald W.</rioxxterms:author><rioxxterms:author>Minaee, Sophie</rioxxterms:author><rioxxterms:author>Mumford, Andrew D.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1111/j.1365-2141.2004.05180.x</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:91
Date: 2017-07-17

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:91</identifier>
      <datestamp>2017-07-17T22:20:50Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Patients with the anti-phospholipid syndrome (APS) have antiphospholipid antibodies (aPA) which are often targeted towards phospholipid binding proteins such as beta2-glycoprotein I and prothrombin. Antibodies to factor XII (FXIIabs) have also been identified in some patients with APS. Factor XII (FXII) is a member of the kringle family of proteins which include plasminogen and prothrombin. Antibodies to prothrombin have been associated with myocardial infarction and have been shown to cross react with plasminogen. Sixteen patients with APS and FXIIabs were investigated for the presence of antibodies to prothrombin, by enzyme linked immunosorbent assay in a calcium (Ca++) independent assay. All sixteen showed different antibody binding patterns than those observed for antibodies to FXII. Eight patients were further investigated using surface plasmon resonance (SPR) for antibody binding to covalently bound FXII and to covalently bound prothrombin in both Ca++ dependent and independent systems. Of three patients demonstrating antibody binding to FXII by SPR, none demonstrated antibody binding to prothrombin in a Ca++ independent system with one demonstrating antibody binding to prothrombin that was Ca++ dependent. Of five patients who did not bind FXII by SPR, one demonstrated antibody binding to prothrombin in a Ca++ independent system while two demonstrated antibody binding to prothrombin in a Ca++ dependent system. Antibodies to FXII in patients with APS appear to be distinct from antibodies to prothrombin.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/91/1/Jones_et_al_2002_Thromb_Haemost_87_426-30.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Chattauer Gmbh-Verlag Medizin Naturwissenschaften, Holderlinstrasse 3, D-70174 Stuttgart, Germany</dc:publisher><dc:source>0340-6245</dc:source><dc:subject>Q</dc:subject><dc:title>Antibodies to factor XII are distinct from antibodies to prothrombin in patients with the anti-phospholipid syndrome.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Jones, David Wynne</rioxxterms:author><rioxxterms:author>Nicholls, Peter J.</rioxxterms:author><rioxxterms:author>Donohoe, S.</rioxxterms:author><rioxxterms:author>Gallimore, Michael J.</rioxxterms:author><rioxxterms:author>Winter, Mark</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2002-03</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:89
Date: 2017-06-29

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:89</identifier>
      <datestamp>2017-06-29T04:52:46Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Forty female patients with either primary anti-phospholipid syndrome (n = 26) or systemic lupus erythematosus (anti-phospholipid syndrome positive) (n = 14) were investigated for levels of factor XII, the presence of lupus anticoagulant and antibodies to cardiolipin, beta 2-glycoprotein I and factor XII. Twenty-one patients had a history of recurrent fetal loss (&gt; 2, mean = 2.6). Lupus anticoagulant positivity showed a weak association with recurrent fetal loss (odds ratio = 1.1). While there was no association between the presence of antibodies to cardiolipin or beta 2-glycoprotein I with recurrent fetal loss, antibodies to factor XII showed a strong and statistically significant association (odds ratio = 5.4, P = 0.025).</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/89/1/Jones_et_al_2001_British_J_of_Haematology_113%2C_550-552.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Wiley</dc:publisher><dc:source>0007-1048</dc:source><dc:subject>Q</dc:subject><dc:title>Antibodies to factor XII and recurrent fetal loss in patients with the anti-phospholipid syndrome.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Jones, David Wynne</rioxxterms:author><rioxxterms:author>Mackie, Ian J.</rioxxterms:author><rioxxterms:author>Gallimore, Michael J.</rioxxterms:author><rioxxterms:author>Winter, Mark</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:88
Date: 2017-01-16

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:88</identifier>
      <datestamp>2017-01-16T23:42:07Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>A molecular screening approach was developed in order to amplify the genomic region that codes for the alpha- and beta-subunits of the nitrile hydratase (NHase) enzyme in rhodococci. Specific PCR primers were designed for the NHase genes from a collection of nitrile-degrading actinomycetes, but amplification was successful only with strains identified as Rhodococcus erythropolis. A hydratase PCR product was also obtained from R. erythropolis DSM 43066(T), which did not grow on nitriles. Southern hybridization of other members of the nitrile-degrading bacterial collection resulted in no positive signals other than those for the R. erythropolis strains used as positive controls. PCR-restriction fragment length polymorphism-single-strand conformational polymorphism (PRS) analysis of the hydratases in the R. erythropolis strains revealed unique patterns that mostly correlated with distinct geographical sites of origin. Representative NHases were sequenced, and they exhibited more than 92.4% similarity to previously described NHases. The phylogenetic analysis and deduced amino acid sequences suggested that the novel R. erythropolis enzymes belonged to the iron-type NHase family. Some different residues in the translated sequences were located near the residues involved in the stabilization of the NHase active site, suggesting that the substitutions could be responsible for the different enzyme activities and substrate specificities observed previously in this group of actinomycetes. A similar molecular screening analysis of the amidase gene was performed, and a correlation between the PRS patterns and the geographical origins identical to the correlation found for the NHase gene was obtained, suggesting that there was coevolution of the two enzymes in R. erythropolis. Our findings indicate that the NHase and amidase genes present in geographically distinct R. erythropolis strains are not globally mixed.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/88/1/Brandao_et_al_2003%2C_Applied_%26_Environ_Micro_69%2C_5754-5766.pdf</dc:identifier><dc:language>en</dc:language><dc:source>Applied and Environmental Microbiology</dc:source><dc:subject>Q</dc:subject><dc:title>Diversity of nitrile hydratase and amidase enzyme genes in Rhodococcus erythropolis recovered from geographically distinct habitats.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Brandao, Pedro F. B.</rioxxterms:author><rioxxterms:author>Clapp, Justin P.</rioxxterms:author><rioxxterms:author>Bull, Alan T.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2003</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1128/AEM.69.10.5754-5766.2003</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:87
Date: 2017-01-16

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:87</identifier>
      <datestamp>2017-01-16T23:46:04Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Bacterial diversity in a deep-sea sediment was investigated by constructing actinobacterium-specific 16S ribosomal DNA (rDNA) clone libraries from sediment sections taken 5 to 12, 15 to 18, and 43 to 46 cm below the sea floor at a depth of 3,814 m. Clones were placed into operational taxonomic unit (OTU) groups with &gt;/= 99% 16S rDNA sequence similarity; the cutoff value for an OTU was derived by comparing 16S rRNA homology with DNA-DNA reassociation values for members of the class Actinobacteria. Diversity statistics were used to determine how the level of dominance, species richness, and genetic diversity varied with sediment depth. The reciprocal of Simpson's index (1/D) indicated that the pattern of diversity shifted toward dominance from uniformity with increasing sediment depth. Nonparametric estimation of the species richness in the 5- to 12-, 15- to 18-, and 43- to 46-cm sediment sections revealed a trend of decreasing species number with depth, 1,406, 308, and 212 OTUs, respectively. Application of the LIBSHUFF program indicated that the 5- to 12-cm clone library was composed of OTUs significantly (P = 0.001) different from those of the 15- to 18- and 43- to 46-cm libraries. F(ST) and phylogenetic grouping of taxa (P tests) were both significant (P &lt; 0.00001 and P &lt; 0.001, respectively), indicating that genetic diversity decreased with sediment depth and that each sediment community harbored unique phylogenetic lineages. It was also shown that even nonconservative OTU definitions result in severe underestimation of species richness; unique phylogenetic clades detected in one OTU group suggest that OTUs do not correspond to real ecological groups sensu Palys (T. Palys, L. K. Nakamura, and F. M. Cohan, Int. J. Syst. Bacteriol. 47:1145-1156, 1997). Mechanisms responsible for diversity and their implications are discussed.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/87/1/Stach_set_al_2003%2C_Appied_%26_Environ_Micro_69%2C_6189-6200.pdf</dc:identifier><dc:language>en</dc:language><dc:source>Applied and Environmental Microbiology</dc:source><dc:subject>Q</dc:subject><dc:title>Statistical approaches for estimating actinobacterial diversity in marine sediments.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Stach, James E.M.</rioxxterms:author><rioxxterms:author>Maldonado, Luis A.</rioxxterms:author><rioxxterms:author>Masson, Douglas G.</rioxxterms:author><rioxxterms:author>Ward, Alan C.</rioxxterms:author><rioxxterms:author>Goodfellow, Michael</rioxxterms:author><rioxxterms:author>Bull, Alan T.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2003</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1128/AEM.69.10.6189-6200.2003</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:86
Date: 2017-01-16

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:86</identifier>
      <datestamp>2017-01-16T23:46:06Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>In this study, we redesigned and evaluated primers for the class Actinobacteria. In silico testing showed that the primers had a perfect match with 82% of genera in the class Actinobacteria, representing a 26-213% improvement over previously reported primers. Only 4% of genera that displayed mismatches did so in the terminal three bases of the 3' end, which is most critical for polymerase chain reaction success. The primers, designated S-C-Act-0235-a-S-20 and S-C-Act-0878-a-A-19, amplified an approximately 640 bp stretch of the 16S rRNA gene from all actinobacteria tested (except Rubrobacter radiotolerans) up to an annealing temperature of 72 degrees C. An Actinobacteria Amplification Resource (http://microbe2.ncl.ac.uk/MMB/AAR.htm) was generated to provide a visual guide to aid the amplification of actinobacterial 16S rDNA. Application of the primers to DNA extracted from marine and terrestrial samples revealed the presence of actinobacteria that have not been described previously. The use of 16S rDNA similarity and DNA-DNA pairing correlations showed that almost every actinomycete clone represented either a new species or a novel genus. The results of this study reinforce the proposition that current culture-based techniques drastically underestimate the diversity of Actinobacteria in the environment and highlight the need to evaluate taxon-specific primers regularly in line with improvements in databases holding 16S rDNA sequences.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/86/1/Stach_et_al_2003%2C_Env_Micro_5%2C_828-841.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Wiley</dc:publisher><dc:source>1462-2912</dc:source><dc:subject>Q</dc:subject><dc:title>New primers for the class Actinobacteria: application to marine and terrestrial environments.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Stach, James E.M.</rioxxterms:author><rioxxterms:author>Maldonado, Luis A.</rioxxterms:author><rioxxterms:author>Ward, Alan C.</rioxxterms:author><rioxxterms:author>Goodfellow, Michael</rioxxterms:author><rioxxterms:author>Bull, Alan T.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2003</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:84
Date: 2017-06-29

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:84</identifier>
      <datestamp>2017-06-29T04:49:21Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/84/1/Bister_et_al_2004_Angew_Chem_Int_Ed_43%2C_2574-2576.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Wiley</dc:publisher><dc:source>1433-7851</dc:source><dc:subject>Q</dc:subject><dc:title>Abyssomicin C-A polycyclic antibiotic from a marine Verrucosispora strain as an inhibitor of the p-aminobenzoic acid/tetrahydrofolate biosynthesis pathway.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Bister, Bojan</rioxxterms:author><rioxxterms:author>Bischoff, Daniel</rioxxterms:author><rioxxterms:author>Strobele, Markus</rioxxterms:author><rioxxterms:author>Riedlinger, Julia</rioxxterms:author><rioxxterms:author>Reicke, Andreas</rioxxterms:author><rioxxterms:author>Wolter, Falko</rioxxterms:author><rioxxterms:author>Bull, Alan T.</rioxxterms:author><rioxxterms:author>Zahner, Hans</rioxxterms:author><rioxxterms:author>Fiedler, Hans-Peter</rioxxterms:author><rioxxterms:author>Sussmuth, Roderich D.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1002/anie.200353160</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:82
Date: 2017-06-30

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:82</identifier>
      <datestamp>2017-06-30T17:27:18Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Although a significant negative prognostic factor, tumor hypoxia can be exploited for gene therapy. To maximize targeting within the tumor mass, we have developed synthetic gene promoters containing hypoxia-responsive elements (HREs) from the erythropoietin (Epo) gene as well as radiation-responsive CArG elements from the early growth response (Egr) 1 gene. Furthermore, to achieve high and sustained expression of the suicide gene herpes simplex virus thymidine kinase (HSVtk), our gene therapy vectors contain an expression amplification system, or 'molecular switch', based on Cre/loxP recombination. In human glioma and breast adenocarcinoma cells exposed to hypoxia and/or radiation, the HRE/CArG promoter rapidly activated Cre recombinase expression leading to selective and sustained HSVtk synthesis. Killing of transfected tumor cells was measured after incubation with the prodrug ganciclovir (GCV; converted by HSVtk into a cytotoxin). In vitro, higher and more selective GCV-mediated toxicity was achieved with the switch vectors, when compared with the same inducible promoters driving HSVtk expression directly. In tumor xenografts implanted in nude mice, the HRE/CArG-switch induced significant growth delay and tumor eradication. In conclusion, hypoxia- and radiation-activated 'molecular switch' vectors represent a promising strategy for both targeted and effective gene therapy of solid tumors.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/82/1/Greco_et_al_2006_Gene_Therapy_206-215.pdf</dc:identifier><dc:language>en</dc:language><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=16307003</dc:relation><dc:source>0969-7128</dc:source><dc:subject>Q</dc:subject><dc:title>Hypoxia- and radiation-activated Cre/loxP 'molecular switch' vectors for gene therapy of cancer.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Greco, Olga</rioxxterms:author><rioxxterms:author>Joiner, Michael C.</rioxxterms:author><rioxxterms:author>Doleh, A.</rioxxterms:author><rioxxterms:author>Powell, A.D.</rioxxterms:author><rioxxterms:author>Hillman, G.G.</rioxxterms:author><rioxxterms:author>Scott, Simon</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2006</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1038/sj.gt.3302640</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:81
Date: 2017-01-17

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
rioxxterms:version_of_record'http://dx.doi.org/10.1038/sj.gt.3302482 ' is not a valid HTTP URI in rioxxterms:version_of_record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
rioxxterms:version_of_record'http://dx.doi.org/10.1038/sj.gt.3302482 ' is not a valid HTTP URI in rioxxterms:version_of_record
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:81</identifier>
      <datestamp>2017-01-17T19:39:34Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132383030</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>During herpes simplex virus type 1 (HSV 1) infection, the tegument protein VP22 is exported from infected cells to the nuclei of surrounding uninfected cells. These intercellular transport characteristics have prompted the exploitation of VP22 fusion proteins for cancer gene therapy, with the goal of maximizing the bystander effect. Since solid tumors contain hypoxic cell populations that are often refractive to therapy, for efficient targeting, it would be optimal if VP22 functioned even at reduced oxygen concentrations. In the present work, VP22 activity under hypoxic conditions was examined for the first time. Plasmid-transfected human glioma U87-MG and U373-MG cells expressing VP22 fused to the green fluorescent protein (GFP) showed protein export to untransfected cells under tumor oxygenation conditions (0-5% O(2)). For suicide gene therapy, VP22 activity was demonstrated under hypoxia by coupling VP22 to the HSV thymidine kinase (HSVtk). In the presence of the prodrug ganciclovir, cell cultures expressing VP22-HSVtk showed a significant increase in toxicity compared with cells transfected with a construct containing HSVtk only, under all tested conditions. To allow effective suicide gene therapy and simultaneous visualization of therapeutic enzyme localization, a triple fusion protein GFP-HSVtk-VP22 was engineered. Functionality of all components was demonstrated under oxia and hypoxia.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/81/1/Greco_et_al_2005_Gene_Therapy_1-6.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Nature Publishing Group</dc:publisher><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=15729368</dc:relation><dc:source>0969-7128</dc:source><dc:subject>Q</dc:subject><dc:title>VP22-mediated intercellular transport for suicide gene therapy under oxic and hypoxic conditions.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Greco, Olga</rioxxterms:author><rioxxterms:author>Joiner, Michael C.</rioxxterms:author><rioxxterms:author>Doleh, A.</rioxxterms:author><rioxxterms:author>Scott, Simon</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2005-06</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1038/sj.gt.3302482 </rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:80
Date: 2017-06-28

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:80</identifier>
      <datestamp>2017-06-28T02:37:52Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Despite being an adverse prognostic factor in radiotherapy, hypoxia represents a physiological difference that can be exploited for selective cancer gene therapy. In this study gene therapy vectors responsive to both hypoxia and ionizing radiation (IR) were developed. Gene expression was regulated by novel, synthetic promoters containing hypoxia responsive elements (HREs) from the erythropoietin (Epo), the phosphoglycerate kinase 1 (PGK1) and the vascular endothelial growth factor (VEGF) genes, and IR-responsive CArG elements from the early growth response (Egr) 1 gene. All chimeric promoters could be activated by hypoxia and/or IR-treatment, and selectively control marker gene expression in human T24 bladder carcinoma and MCF-7 mammary carcinoma cells. Importantly, enhancers containing combinations of HREs and CArG elements were able to respond to both triggering treatments, with the Epo HRE/CArG combination proving to be the most responsive and robust. The Epo HRE/CArG enhancer could effectively control a suicide gene therapy strategy by selectively sensitizing hypoxic and/or irradiated cells expressing the enzyme horseradish peroxidase (HRP) to the prodrug indole-3-acetic acid (IAA). These data indicate that the use of such chimeric promoters may effectively regulate therapeutic gene expression within the tumor microenvironment in gene therapy strategies aimed at addressing the problem of hypoxia in radiotherapy.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/80/1/Greco_et_al_2002_Gene_Therapy_2002%2C_1403-1411.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Elsevier Science BV, Sara Burgerhartstraat 25, PO Box 211, 1000 AE Amsterdam, Netherlands</dc:publisher><dc:source>0167-2738</dc:source><dc:subject>Q</dc:subject><dc:title>Novel chimeric gene promoters responsive to hypoxia and ionizing radiation.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Greco, Olga</rioxxterms:author><rioxxterms:author>Marples, Brian</rioxxterms:author><rioxxterms:author>Dachs, G.U.</rioxxterms:author><rioxxterms:author>Williams, Kaye J.</rioxxterms:author><rioxxterms:author>Patterson, Adam V.</rioxxterms:author><rioxxterms:author>Scott, Simon</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2002-10</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:79
Date: 2017-06-26

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:79</identifier>
      <datestamp>2017-06-26T17:22:55Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>We have been developing synthetic gene promoters responsive to clinical doses of ionizing radiation (IR) for use in suicide gene therapy vectors. The crucial DNA sequences utilized are units with the consensus motif CC(A/T)(6)GG, known as CArG elements, derived from the IR-responsive Egr1 gene. In this study we have investigated the parameters needed to enhance promoter activation to radiation. A series of plasmid vectors containing different enhancer/promoters were constructed, transiently transfected into tumor cells (MCF-7 breast adenocarcinoma and U-373MG glioblastoma) and expression of a downstream reporter assayed. Results revealed that increasing the number of CArG elements, up to a certain level, increased promoter radiation-response; from a fold-induction of 1.95 +/- 0.17 for four elements to 2.74 +/- 0.17 for nine CArGs of the same sequence (for MCF-7 cells). Specific alteration of the core A/T sequences caused an even greater positive response, with fold-inductions of 1.71 +/- 0.23 for six elements of prototype sequence compared with 2.96 +/- 0.52 for one of the new sequences following irradiation. Alteration of spacing (from six to 18 nucleotides) between elements had little effect, as did the addition of an adjacent Sp1 binding site. Combining the optimum number and sequence of CArG elements in an additional enhancer was found to produce the best IR induction levels. Furthermore, the improved enhancers also performed better than the previously reported prototype when used in in vitro and in vivo experimental GDEPT. We envisage such enhancers will be used to drive suicide gene expression from vectors delivered to a tumor within an irradiated field. The modest, but tight expression described in the present study could be amplified using a molecular 'switch' system as previously described using Cre/LoxP. In combination with targeted delivery, this strategy has great potential for significantly improving the efficacy of cancer treatment in the large number of cases where radiotherapy is currently employed.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/79/1/Scott_et_al_2002_Gene_Therapy_9%2C_1396-1402.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Nature Publishing Group,Macmillan Building, 4 Crinan St,London N1 9Xw ,England </dc:publisher><dc:source>0969-7128</dc:source><dc:subject>Q</dc:subject><dc:title>Optimizing radiation-responsive gene promoters for radiogenetic cancer therapy.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Scott, Simon</rioxxterms:author><rioxxterms:author>Joiner, Michael C.</rioxxterms:author><rioxxterms:author>Marples, Brian</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2002-10</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1038/sj.gt.3301822</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:78
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:78</identifier>
      <datestamp>2016-04-27T15:03:42Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The secreted Mycobacterium tuberculosis complex proteins CFP-10 and ESAT-6 have recently been shown to play an essential role in tuberculosis pathogenesis. We have determined the solution structure of the tight, 1:1 complex formed by CFP-10 and ESAT-6, and employed fluorescence microscopy to demonstrate specific binding of the complex to the surface of macrophage and monocyte cells. A striking feature of the complex is the long flexible arm formed by the C-terminus of CFP-10, which was found to be essential for binding to the surface of cells. The surface features of the CFP-10.ESAT-6 complex, together with observed binding to specific host cells, strongly suggest a key signalling role for the complex, in which binding to cell surface receptors leads to modulation of host cell behaviour to the advantage of the pathogen.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/78/1/Renshaw_et_al_EMBO_J_2005.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>EMBO Press</dc:publisher><dc:source>0261-4189</dc:source><dc:subject>Q</dc:subject><dc:title>Structure and function of the complex formed by the tuberculosis virulence factors CFP-10 and ESAT-6.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Renshaw, Philip S.</rioxxterms:author><rioxxterms:author>Lightbody, Kirsty L.</rioxxterms:author><rioxxterms:author>Veverka, Vaclav</rioxxterms:author><rioxxterms:author>Muskett, Frederick W.</rioxxterms:author><rioxxterms:author>Kelly, Geoff</rioxxterms:author><rioxxterms:author>Frenkiel, Tom A.</rioxxterms:author><rioxxterms:author>Gordon, Stephen V.</rioxxterms:author><rioxxterms:author>Hewinson, R. Glyn</rioxxterms:author><rioxxterms:author>Burke, Bernard</rioxxterms:author><rioxxterms:author>Norman, Jim</rioxxterms:author><rioxxterms:author>Williamson, Richard A.</rioxxterms:author><rioxxterms:author>Carr, Mark D.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2005</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:77
Date: 2017-01-16

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:77</identifier>
      <datestamp>2017-01-16T16:37:19Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The closely related mycobacteria responsible for tuberculosis produce an unusually high number of secreted proteins, many of which are clearly implicated in pathogenesis and protective immunity. Falling within this category are the closely related proteins MPB70 and MPB83. The structure of MPB70 reveals a complex and novel bacterial fold, which has clear structural homology to the two C-terminal FAS1 domains of the cell adhesion protein fasciclin I, whose structures were reported very recently. Assessment of the surface features of MPB70, the sequence divergence between MPB70 and MPB83, the conservation of residues across a group of FAS1 domains, and the locations of disease-inducing mutations in betaig-h3 strongly suggests that MPB70 and MPB83 contain two functional surfaces on opposite faces, which are probably involved in binding to host cell proteins. This analysis also suggests that these functional surfaces are retained in the FAS1 proteins associated with mediating interactions between cells and the extracellular matrix (fasciclin I, periostin, and betaig-h3) and furthermore that some of the human corneal disease-inducing substitutions identified in betaig-h3 will perturb interactions at these sites.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/77/1/Carr_et_al_2003%2C_J_Biol_Chem_278%2C_43736-43743pdf.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Society for Biochemistry and Molecular Biology</dc:publisher><dc:source>0021-9258</dc:source><dc:subject>Q</dc:subject><dc:title>Solution structure of the Mycobacterium tuberculosis complex protein MPB70: from tuberculosis pathogenesis to inherited human corneal desease.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Carr, Mark D.</rioxxterms:author><rioxxterms:author>Bloemink, Marieke J.</rioxxterms:author><rioxxterms:author>Dentten, Ellen</rioxxterms:author><rioxxterms:author>Whelan, Adam</rioxxterms:author><rioxxterms:author>Gordon, Stephen V.</rioxxterms:author><rioxxterms:author>Kelly, Geoff</rioxxterms:author><rioxxterms:author>Frenkiel, Tom A.</rioxxterms:author><rioxxterms:author>Hewinson, R. Glyn</rioxxterms:author><rioxxterms:author>Williamson, Richard A.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2003</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:76
Date: 2017-06-29

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:76</identifier>
      <datestamp>2017-06-29T13:58:05Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Tissue inhibitor of metalloproteinases-2 (TIMP-2) is unique as it is the only member of the TIMP family that is involved in the cellular activation of promatrix metalloproteinase-2 (pro-MMP-2) by virtue of forming a trimolecular complex with membrane type 1 matrix metalloproteinase (MT1-MMP) on the cell surface. TIMP-4 is similar in structure to TIMP-2 but is unable to support the activation of the proenzyme. Several reports have highlighted the importance of the TIMP-2 C-terminal domain in the pro-MMP-2 activation complex; however, very little is known about the role of the extended AB loop of TIMP-2 in this mechanism even though it has been shown to interact with MT1-MMP. In this study we show by mutagenesis and kinetic analysis that it is possible to transfer the MT1-MMP binding affinity of the TIMP-2 AB loop to TIMP-4 but that its transplantation into TIMP-4 does not endow the inhibitor with pro-MMP-2 activating activity. However, transfer of both the AB loop and C-terminal domain of TIMP-2 to TIMP-4 generates a mutant that can activate pro-MMP-2 and so demonstrates that both these regions of TIMP-2 are important for the activation process.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/76/1/Rapti_et_al_JBC_2006_23386-23394.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Society for Biochemistry and Molecular Biology</dc:publisher><dc:source>0021-9258</dc:source><dc:subject>Q</dc:subject><dc:title>Characterization of the AB loop region of TIMP-2. Involvement in pro-MMP-2 activation.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Rapti, Magdalini</rioxxterms:author><rioxxterms:author>Knauper, Vera</rioxxterms:author><rioxxterms:author>Murphy, Gillian</rioxxterms:author><rioxxterms:author>Williamson, Richard A.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2006</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:75
Date: 2017-06-28

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:75</identifier>
      <datestamp>2017-06-28T08:08:14Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The tissue inhibitor of metalloproteinases-2 (TIMP-2) is potentially an important inhibitor of all known matrix metalloproteinases (MMPs). However, it has been shown to undergo specific interactions with both MMP-2 (gelatinase A) and MMP-14 (MT1-MMP), and it has been proposed that these three proteins function as a cell surface-based activation cascade for matrix metalloproteinases and as a focus of proteolytic activity. In this study, we have carried out mutagenesis and kinetic analyses to examine the unique interactions between the AB loop of TIMP-2 and MMP-14. The results demonstrate that the major binding contribution of the AB loop is due solely to residue Tyr-36 at the tip of the hairpin. From this work, we propose that TIMP-2 may be engineered to abrogate MMP-14 binding, whereas its binding properties for other MMPs, including MMP-2, are maintained. Mutants of TIMP-2 with more directed specificity may be of use in gene therapeutic approaches to human disease.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/75/1/Williamson_et_al_2001%2C_J_Biol_Chem_276%2C_32966-32970.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Society for Biochemistry and Molecular Biology</dc:publisher><dc:source>0021-9258</dc:source><dc:subject>Q</dc:subject><dc:title>Tyrosine 36 plays a critical role in the interaction of the AB loop of tissue inhibitor of metalloproteinases-2 with matrix metalloproteinase-14.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Williamson, Richard A.</rioxxterms:author><rioxxterms:author>Hutton, Mike</rioxxterms:author><rioxxterms:author>Vogt, Gavin</rioxxterms:author><rioxxterms:author>Rapti, Magdalene</rioxxterms:author><rioxxterms:author>Knauper, Vera</rioxxterms:author><rioxxterms:author>Carr, Mark D.</rioxxterms:author><rioxxterms:author>Murphy, Gillian</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:74
Date: 2017-01-14

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:74</identifier>
      <datestamp>2017-01-14T23:57:22Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>One of the most intriguing steps during cobalamin (vitamin B12) biosynthesis is the ring contraction process that leads to the extrusion of one of the integral macrocyclic carbon atoms from the tetrapyrrole-derived framework. The aerobic cobalamin pathway requires the action of a monooxygenase called CobG (precorrin-3B synthase), which generates a hydroxylactone intermediate that is subsequently ring-contracted by CobJ. However, in the photosynthetic bacterium Rhodobacter capsulatus, which harbors an aerobic-like pathway, there is no cobG in the main cobalamin biosynthetic operon although it does contain an additional uncharacterized gene called orf663. To demonstrate the involvement of Orf663 in cobalamin synthesis, the first dedicated 10 genes of the B12 pathway (including orf663), encoding enzymes for the transformation of uroporphyrinogen III into hydrogenobyrinic acid (HBA), were sequentially cloned into a plasmid to generate an artificial operon, which, when transformed into Escherichia coli, endowed the host with the ability to make HBA. Deletion of orf663 from this operon prevented HBA synthesis, demonstrating that it was essential for corrin construction. HBA synthesis was restored to this recombinant strain either by returning orf663 or by substituting it with cobG. Recombinant overproduction of Orf663, now renamed CobZ, allowed the characterization of a novel cofactor-rich protein, housing two Fe-S centers, a flavin, and a heme group, which like B12 itself is a modified tetrapyrrole. A mechanism for Orf663 (CobZ) in cobalamin biosynthesis is proposed.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/74/1/McGoldrick_et_al_2005%2C_J_Biol_Chem_280%2C_1086-94.pdf</dc:identifier><dc:language>en</dc:language><dc:source>Journal of Biological Chemistry</dc:source><dc:subject>Q</dc:subject><dc:title>Identification and characterization of a novel vitamin B12 (cobalamin) biosynthetic enzyme (CobZ) from Rhodobacter capsulatus, containing flavin, heme, and Fe-S cofactors.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>McGoldrick, Helen M.</rioxxterms:author><rioxxterms:author>Roessners, Charles A.</rioxxterms:author><rioxxterms:author>Raux-Deery, Evelyne</rioxxterms:author><rioxxterms:author>Lawrence, Andrew D.</rioxxterms:author><rioxxterms:author>McLean, Kirsty J.</rioxxterms:author><rioxxterms:author>Munro, Andrew W.</rioxxterms:author><rioxxterms:author>Santabarbara, Stefano</rioxxterms:author><rioxxterms:author>Rigby, Stephen E. J.</rioxxterms:author><rioxxterms:author>Heathcote, Peter</rioxxterms:author><rioxxterms:author>Scott, A. Ian</rioxxterms:author><rioxxterms:author>Warren, Martin J.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2005</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:73
Date: 2017-01-14

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:73</identifier>
      <datestamp>2017-01-14T23:57:09Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Vitamin B12 (cobalamin) was identified nearly 80 years ago as the anti-pernicious anaemia factor in liver, and its importance in human health and disease has resulted in much work on its uptake, cellular transport and utilization. Plants do not contain cobalamin because they have no cobalamin-dependent enzymes. Deficiencies are therefore common in strict vegetarians, and in the elderly, who are susceptible to an autoimmune disorder that prevents its efficient uptake. In contrast, many algae are rich in vitamin B12, with some species, such as Porphyra yezoensis (Nori), containing as much cobalamin as liver. Despite this, the role of the cofactor in algal metabolism remains unknown, as does the source of the vitamin for these organisms. A survey of 326 algal species revealed that 171 species require exogenous vitamin B12 for growth, implying that more than half of the algal kingdom are cobalamin auxotrophs. Here we show that the role of vitamin B12 in algal metabolism is primarily as a cofactor for vitamin B12-dependent methionine synthase, and that cobalamin auxotrophy has arisen numerous times throughout evolution, probably owing to the loss of the vitamin B12-independent form of the enzyme. The source of cobalamin seems to be bacteria, indicating an important and unsuspected symbiosis.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/73/1/Croft_et_al_2005%2C_Nature_438%2C_90-93.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Nature Publishing Group</dc:publisher><dc:source>0028-0836</dc:source><dc:subject>Q</dc:subject><dc:title>Algae acquire vitamin B12 through a symbiotic relationship with bacteria.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Croft, Martin T.</rioxxterms:author><rioxxterms:author>Lawrence, Andrew D.</rioxxterms:author><rioxxterms:author>Raux-Deery, Evelyne</rioxxterms:author><rioxxterms:author>Warren, Martin J.</rioxxterms:author><rioxxterms:author>Smith, Alison G.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2005</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1038/nature04056</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:72
Date: 2017-01-14

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:72</identifier>
      <datestamp>2017-01-14T23:54:58Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>In 1969 it was proposed that the episodic madness suffered by King George III (1738-1820) resulted from an acute hereditary porphyria, variegate porphyria, caused by deficiency of protoporphyrinogen oxidase. The diagnosis was based on the historical archive and a contentious claim that living members of the House of Hanover were affected with the condition. A re-examination of the medical evidence and the appearance of new historical material have suggested that porphyria did indeed exist in the Royal Houses of Europe. We report the analysis of hair obtained from George III. Although no genomic DNA could be obtained, metal analysis revealed high concentrations of arsenic. Since arsenic interferes with haem metabolism, it might have contributed to the King's unusually severe and prolonged bouts of illness. We have identified sources of arsenic in the context of the medication George III received from physicians.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/72/1/Cox_et_al_2005%2C_The_Lancet%2C_366%2C_332-335.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Elsevier</dc:publisher><dc:source>0140-6736</dc:source><dc:subject>Q</dc:subject><dc:title>King George III and porphyria: an elemental hypothesis and investigation.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Cox, Timothy M.</rioxxterms:author><rioxxterms:author>Jack, Nicola</rioxxterms:author><rioxxterms:author>Lofthouse, Simon</rioxxterms:author><rioxxterms:author>Watling, John</rioxxterms:author><rioxxterms:author>Haines, Janice</rioxxterms:author><rioxxterms:author>Warren, Martin J.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2005</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:71
Date: 2017-06-26

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:71</identifier>
      <datestamp>2017-06-26T17:27:03Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Sirohaem is a tetrapyrrole-derived prosthetic group that is required for the essential assimilation of sulfur and nitrogen into all living systems as part of the sulfite and nitrite reductase systems. The final two steps in the biosynthesis of sirohaem involve a beta-NAD(+)-dependent dehydrogenation of precorrin-2 to generate sirohydrochlorin followed by ferrochelation to yield sirohaem. In Saccharomyces cerevisiae, Met8p is a bifunctional enzyme that carries out both of these reactions. Here, we report the 2.2 A resolution crystal structure of Met8p, which adopts a novel fold that bears no resemblance to the previously determined structures of cobalt- or ferro-chelatases. Analysis of mutant proteins suggests that both catalytic activities share a single active site, and that Asp141 plays an essential role in both dehydrogenase and chelatase processes.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/71/1/Schubert_et_al_2002%2C_EMBO_J_21%2C_2068-2075.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>EMBO Press</dc:publisher><dc:source>0261-4189</dc:source><dc:subject>Q</dc:subject><dc:title>The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Schubert, Heidi L.</rioxxterms:author><rioxxterms:author>Raux-Deery, Evelyne</rioxxterms:author><rioxxterms:author>Brindley, Amanda A.</rioxxterms:author><rioxxterms:author>Leech, Helen K.</rioxxterms:author><rioxxterms:author>Wilson, Keith S.</rioxxterms:author><rioxxterms:author>Hill, Christopher P.</rioxxterms:author><rioxxterms:author>Warren, Martin J.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2002</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:70
Date: 2016-01-06

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:70</identifier>
      <datestamp>2016-01-06T10:14:24Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><ali:free_to_read/><dc:description>The cap-binding protein eIF4E is the first in a chain of translation initiation factors that recruit 40S ribosomal subunits to the 5' end of eukaryotic mRNA. During cap-dependent translation, this protein binds to the 5'-terminal m(7)Gppp cap of the mRNA, as well as to the adaptor protein eIF4G. The latter then interacts with small ribosomal subunit-bound proteins, thereby promoting the mRNA recruitment process. Here, we show apo-eIF4E to be a protein that contains extensive unstructured regions, which are induced to fold upon recognition of the cap structure. Binding of eIF4G to apo-eIF4E likewise induces folding of the protein into a state that is similar to, but not identical with, that of cap-bound eIF4E. At the same time, binding of each of the binding partners of eIF4E modulates the kinetics with which it interacts with the other partner. We present structural, kinetic and mutagenesis data that allow us to deduce some of the detailed folding transitions that take place during the eIF4E interactions.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/70/1/von_der_Haar_et_al_2005.pdf</dc:identifier><dc:language>en</dc:language><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=16405910</dc:relation><dc:source>Journal of Molecular Biology</dc:source><dc:subject>Q</dc:subject><dc:title>Folding transitions during assembly of the eukaryotic mRNA cap-binding complex.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>von der Haar, Tobias</rioxxterms:author><rioxxterms:author>Oku, Yuko</rioxxterms:author><rioxxterms:author>Ptushkina, Marina</rioxxterms:author><rioxxterms:author>Moerke, Nathan</rioxxterms:author><rioxxterms:author>Wagner, Gerhard</rioxxterms:author><rioxxterms:author>Gross, John D.</rioxxterms:author><rioxxterms:author>McCarthy, John E. G.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2006</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1016/j.jmb.2005.12.034</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:69
Date: 2017-01-17

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:69</identifier>
      <datestamp>2017-01-17T03:02:20Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Eukaryotic initiation factor 4G (eIF4G) promotes mRNA recruitment to the ribosome by binding to the mRNA cap- and poly(A) tail-binding proteins eIF4E and Pap1p. eIF4G also binds eIF4A at a distinct HEAT domain composed of five stacks of antiparallel alpha-helices. The role of eIF4G in the later steps of initiation, such as scanning and AUG recognition, has not been defined. Here we show that the entire HEAT domain and flanking residues of Saccharomyces cerevisiae eIF4G2 are required for the optimal interaction with the AUG recognition factors eIF5 and eIF1. eIF1 binds simultaneously to eIF4G and eIF3c in vitro, as shown previously for the C-terminal domain of eIF5. In vivo, co-overexpression of eIF1 or eIF5 reverses the genetic suppression of an eIF4G HEAT domain Ts(-) mutation by eIF4A overexpression. In addition, excess eIF1 inhibits growth of a second eIF4G mutant defective in eIF4E binding, which was also reversed by co-overexpression of eIF4A. Interestingly, excess eIF1 carrying the sui1-1 mutation, known to relax the accuracy of start site selection, did not inhibit the growth of the eIF4G mutant, and sui1-1 reduced the interaction between eIF4G and eIF1 in vitro. Moreover, a HEAT domain mutation altering eIF4G moderately enhances translation from a non-AUG codon. These results strongly suggest that the binding of the eIF4G HEAT domain to eIF1 and eIF5 is important for maintaining the integrity of the scanning ribosomal preinitiation complex.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/69/1/He_et_al_2003%2C_Mol_Cell_Biol_23%2C_5431-5445.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Society for Microbiology</dc:publisher><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=12861028</dc:relation><dc:source>0270-7306</dc:source><dc:subject>Q</dc:subject><dc:title>The yeast eukaryotic initiation factor 4G (eIF4G) HEAT domain interacts with eIF1 and eIF5 and is involved in stringent AUG selection.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>He, Hui</rioxxterms:author><rioxxterms:author>von der Haar, Tobias</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2003</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1128/MCB.23.15.5431-5445.2003</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:68
Date: 2017-06-29

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:68</identifier>
      <datestamp>2017-06-29T04:51:24Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Knowledge of the balance of activities of eukaryotic initiation factors (eIFs) is critical to our understanding of the mechanisms underlying translational control. We have therefore estimated the intracellular levels of 11 eIFs in logarithmically growing cells of Saccharomyces cerevisiae using polyclonal antibodies raised in rabbits against recombinant proteins. Those factors involved in 43S complex formation occur at levels comparable (i.e. within a 0.5- to 2.0-fold range) to those published for ribosomes. In contrast, the subunits of the cap-binding complex eIF4F showed considerable variation in their abundance. The helicase eIF4A was the most abundant eIF of the yeast cell, followed by eIF4E at multiple copies per ribosome, and eIF4B at approximately one copy per ribosome. The adaptor protein eIF4G was the least abundant of the eIF4 factors, with a copy number per cell that is substoichiometric to the ribosome and similar to the abundance of mRNA. The observed excess of eIF4E over its functional partner eIF4G is not strictly required during exponential growth: at eIF4E levels artificially reduced to 30% of those in wild-type yeast, growth rates and the capacity for general protein synthesis are only minimally affected. This demonstrates that eIF4E does not exercise a higher level of rate control over translation than other eIFs. However, other features of the yeast life cycle, such as the control of cell size, are more sensitive to changes in eIF4E abundance. Overall, these data constitute an important basis for developing a quantitative model of the workings of the eukaryotic translation apparatus.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/68/1/von_der_Haar_2002%2C_Mol_Microbiol_46%2C_531-544.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Wiley</dc:publisher><dc:source>0950-382X</dc:source><dc:subject>Q</dc:subject><dc:title>Intracellular translation initiation factor levels in Saccharomyces cerevisiae and their role in cap-complex function.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>von der Haar, Tobias</rioxxterms:author><rioxxterms:author>McCarthy, John E. G.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2002</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:67
Date: 2017-01-16

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:67</identifier>
      <datestamp>2017-01-16T22:18:01Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The yeast Saccharomyces cerevisiae genome encodes several proteins that, in laboratory strains, can take up a stable, transmissible prion form. In each case, this requires the Asn/Gln-rich prion-forming domain (PrD) of the protein to be intact. In order to further understand the evolutionary significance of this unusual property, we have examined four different prion genes and their corresponding PrDs, from a number of naturally occurring strains of S. cerevisiae. In 4 of the 16 strains studied we identified a new allele of the SUP35 gene (SUP35delta19) that contains a 19-amino-acid deletion within the N-terminal PrD, a deletion that eliminates the prion property of Sup35p. In these strains a second prion gene, RNQ1, was found to be highly polymorphic, with eight different RNQ1 alleles detected in the six diploid strains studied. In contrast, for one other prion gene (URE2) and the sequence of the NEW1 gene encoding a PrD, no significant degree of DNA polymorphism was detected. Analysis of the naturally occurring alleles of RNQ1 and SUP35 indicated that the various polymorphisms identified were associated with DNA tandem repeats (6, 12, 33, 42 or 57 bp) within the coding sequences. The expansion and contraction of DNA repeats within the RNQ1 gene may provide an evolutionary mechanism that can ensure rapid change between the [PRION+] and [prion-] states.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/67/1/Resende_et_al_2003%2C_Molecular_Microbiology_49%2C_1005-1017.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Great Britain : Blackwell Publishing Ltd</dc:publisher><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=12890024</dc:relation><dc:source>0950-382X</dc:source><dc:subject>Q</dc:subject><dc:title>Prion protein gene polymorphisms in Saccharomyces cerevisiae.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Resende, Catarina</rioxxterms:author><rioxxterms:author>Outeiro, Tiago F.</rioxxterms:author><rioxxterms:author>Sands, Laina</rioxxterms:author><rioxxterms:author>Lindquist, Susan L.</rioxxterms:author><rioxxterms:author>Tuite, Mick F.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2003</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1046/j.1365-2958.2003.03608.x</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:66
Date: 2017-01-12

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:66</identifier>
      <datestamp>2017-01-12T19:10:53Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><ali:free_to_read/><dc:description>Many proteins can misfold into beta-sheet-rich, self-seeding polymers (amyloids). Prions are exceptional among such aggregates in that they are also infectious. In fungi, prions are not pathogenic but rather act as epigenetic regulators of cell physiology, providing a powerful model for studying the mechanism of prion replication. We used prion-forming domains from two budding yeast proteins (Sup35p and New1p) to examine the requirements for prion formation and inheritance. In both proteins, a glutamine/asparagine-rich (Q/N-rich) tract mediates sequence-specific aggregation, while an adjacent motif, the oligopeptide repeat, is required for the replication and stable inheritance of these aggregates. Our findings help to explain why although Q/N-rich proteins are relatively common, few form heritable aggregates: prion inheritance requires both an aggregation sequence responsible for self-seeded growth and an element that permits chaperone-dependent replication of the aggregate. Using this knowledge, we have designed novel artificial prions by fusing the replication element of Sup35p to aggregation-prone sequences from other proteins, including pathogenically expanded polyglutamine.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/66/1/Osherovich_et_al_2004%2C_PLoS_Biology_2%2C_442-451.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Public Library of Science</dc:publisher><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=15045026</dc:relation><dc:source>1544-9173</dc:source><dc:subject>Q</dc:subject><dc:title>Dissection and design of yeast prions.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Osherovich, Lev Z.</rioxxterms:author><rioxxterms:author>Cox, Brian S.</rioxxterms:author><rioxxterms:author>Tuite, Mick F.</rioxxterms:author><rioxxterms:author>Weissman, Jonathan S.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004-03-23</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1371/journal.pbio.0020086</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:65
Date: 2017-01-16

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:65</identifier>
      <datestamp>2017-01-16T14:07:34Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>[PSI(+)] strains of the yeast Saccharomyces cerevisiae replicate and transmit the prion form of the Sup35p protein but can be permanently cured of this property when grown in millimolar concentrations of guanidine hydrochloride (GdnHCl). GdnHCl treatment leads to the inhibition of the replication of the [PSI(+)] seeds necessary for continued [PSI(+)] propagation. Here we demonstrate that the rate of incorporation of newly synthesized Sup35p into the high-molecular-weight aggregates, diagnostic of [PSI(+)] strains, is proportional to the number of seeds in the cell, with seed number declining (and the levels of soluble Sup35p increasing) in the presence of GdnHCl. GdnHCl does not cause breakdown of preexisting Sup35p aggregates in [PSI(+)] cells. Transfer of GdnHCl-treated cells to GdnHCl-free medium reverses GdnHCl inhibition of [PSI(+)] seed replication and allows new prion seeds to be generated exponentially in the absence of ongoing protein synthesis. Following such release the [PSI(+)] seed numbers double every 20 to 22 min. Recent evidence (P. C. Ferreira, F. Ness, S. R. Edwards, B. S. Cox, and M. F. Tuite, Mol. Microbiol. 40:1357-1369, 2001; G. Jung and D. C. Masison, Curr. Microbiol. 43:7-10, 2001), together with data presented here, suggests that curing yeast prions by GdnHCl is a consequence of GdnHCl inhibition of the activity of molecular chaperone Hsp104, which in turn is essential for [PSI(+)] propagation. The kinetics of elimination of [PSI(+)] by coexpression of a dominant, ATPase-negative allele of HSP104 were similar to those observed for GdnHCl-induced elimination. Based on these and other data, we propose a two-cycle model for "prionization" of Sup35p in [PSI(+)] cells: cycle A is the GdnHCl-sensitive (Hsp104-dependent) replication of the prion seeds, while cycle B is a GdnHCl-insensitive (Hsp104-independent) process that converts these seeds to pelletable aggregates.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/65/1/Ness_et_al_2002%2C_Mol_Cell_Biol_22%2C_5593-5605.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Society of MicroBiology </dc:publisher><dc:relation>http://mcb.asm.org/cgi/content/abstract/22/15/5593</dc:relation><dc:source>0270-7306 </dc:source><dc:subject>Q</dc:subject><dc:title>Guanidine hydrochloride inhibits the generation of prion "seeds" but not prion protein aggregation in yeast.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Ness, Frederique</rioxxterms:author><rioxxterms:author>Ferreira, Paulo C.</rioxxterms:author><rioxxterms:author>Cox, Brian S.</rioxxterms:author><rioxxterms:author>Tuite, Mick F.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2002-08</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1128/MCB.22.15.5593-5605.2002</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:64
Date: 2017-01-16

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:64</identifier>
      <datestamp>2017-01-16T10:32:01Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The nuclear-encoded Sup35p protein is responsible for the prion-like [PSI(+)] determinant of yeast, with Sup35p existing largely as a high molecular weight aggregate in [PSI(+)] strains. Here we show that the five oligopeptide repeats present at the N-terminus of Sup35p are responsible for stabilizing aggregation of Sup35p in vivo. Sequential deletion of the oligopeptide repeats prevented the maintenance of [PSI(+)] by the truncated Sup35p, although deletants containing only two repeats could be incorporated into pre-existing aggregates of wild-type Sup35p. The mammalian prion protein PrP also contains similar oligopeptide repeats and we show here that a human PrP repeat (PHGGGWGQ) is able functionally to replace a Sup35p oligopeptide repeat to allow stable [PSI(+)] propagation in vivo. Our data suggest a model in which the oligopeptide repeats in Sup35p stabilize intermolecular interactions between Sup35p proteins that initiate establishment of the aggregated state. Modulating repeat number therefore alters the rate of yeast prion conversion in vivo. Furthermore, there appears to be evolutionary conservation of function of the N-terminally located oligopeptide repeats in prion propagation.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/64/1/Parham_et_al_2001%2C_EMBO_J_20%2C_2111-2119.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Oxford University Press</dc:publisher><dc:source>0261-4189 </dc:source><dc:subject>Q</dc:subject><dc:title>Oligopeptide repeats in the yeast protein Sup35p stabilize intermolecular prion interactions.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Parham, Steven N.</rioxxterms:author><rioxxterms:author>Resende, Catarina</rioxxterms:author><rioxxterms:author>Tuite, Mick F.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001-05-01</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1093/emboj/20.9.2111</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:62
Date: 2016-11-15

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:62</identifier>
      <datestamp>2016-11-15T12:02:53Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>To ensure the optimal safety of plasma derived and new generation recombinant proteins, heat treatment is customarily applied in the manufacturing of such biopharmaceuticals as a means of viral inactivation. In subjecting proteins to anti-viral heat-treatment it is necessary to use high concentrations of thermostabilizing excipients to prevent protein damage, and it is therefore imperative that the correct balance between bioprocessing conditions, maintenance of protein integrity and virus kill is found. In this study we have utilized model proteins (lysozyme, fetuin, and human serum albumin) and plasma-derived therapeutic proteins (factor VIII and factor IX) to investigate the protein modifications that occur during anti-viral heat treatment. Specifically, we investigated the relationship between bioprocessing conditions and the type and extent of protein modification under a variety of industrially relevant wet and lyophilized heat treatments using sucrose as a thermostabilizing agent. Heat treatment led to the formation of disulfide crosslinks and aggregates in proteins containing free cysteine residues. Terminal oligosaccharide sialic acid residues were hydrolyzed from the glycan moieties of glycoproteins during anti-viral heat treatment. Heat treatment promoted sucrose hydrolysis to yield glucose and fructose, leading, in turn, to the glycation of lysine amino groups in those proteins containing di-lysine motifs. During extended hear treatments, 1,2-dicarbonyl type advanced glycation end-products were also formed. Glycation-type modifications were more prevalent in wet heat-treated protein formulations.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/62/1/Smales_et_al_2002%2C_Biotechnology_%26_Bioengineering_77.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>John Wiley &amp; Sons LTD, 111 River Street, Hoboken, NJ 07030 USA</dc:publisher><dc:source>0006-3592</dc:source><dc:subject>Q</dc:subject><dc:title>Protein modification during anti-viral heat-treatment bioprocessing of factor VIII concentrates, factor IX concentrates, and model proteins in the presence of sucrose.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Smales, C. Mark</rioxxterms:author><rioxxterms:author>Pepper, Duncan S.</rioxxterms:author><rioxxterms:author>James, David C.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2002-01-05</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1002/bit.10161</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:61
Date: 2017-01-18

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:61</identifier>
      <datestamp>2017-01-18T14:13:46Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Listeria monocytogenes is a food-borne Gram-positive bacterium that is responsible for a variety of infections (worldwide) annually. The organism is able to survive a variety of environmental conditions and stresses, however, the mechanisms by which L. monocytogenes adapts to environmental change are yet to be fully elucidated. An understanding of the mechanism(s) by which L. monocytogenes survives unfavourable environmental conditions will aid in developing new food processing methods to control the organism in foodstuffs. We have utilized a proteomic approach to investigate the response of L. monocytogenes batch cultures to the transition from exponential to stationary growth phase. Proteomic analysis showed that batch cultures of L. monocytogenes perceived stress and began preparations for stationary phase much earlier (approximately A(600) = 0.75, mid-exponential) than predicted by growth characteristics alone. Global analysis of the proteome revealed that the expression levels of more than 50% of all proteins observed changed significantly over a 7-9 h period during this transition phase. We have highlighted ten proteins in particular whose expression levels appear to be important in the early onset of the stationary phase. The significance of these findings in terms of functionality and the mechanistic picture are discussed.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/61/1/Weeks_et_al_2004%2C_Proteomics_4%2C_123-135.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>WILEY-V C H VERLAG GMBH, PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY </dc:publisher><dc:source>1615-9853</dc:source><dc:subject>Q</dc:subject><dc:title>Global changes in gene expression observed at the transition from growth to stationary phase in Listeria monocytogenes ScottA batch culture</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Weeks, Mark E.</rioxxterms:author><rioxxterms:author>James, David C.</rioxxterms:author><rioxxterms:author>Robinson, Gary K.</rioxxterms:author><rioxxterms:author>Smales, C. Mark</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1002/pmic.200300527</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:60
Date: 2016-11-15

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:60</identifier>
      <datestamp>2016-11-15T12:02:32Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>We have employed an inverse engineering strategy based on quantitative proteome analysis to identify changes in intracellular protein abundance that correlate with increased specific recombinant monoclonal antibody production (qMab) by engineered murine myeloma (NS0) cells. Four homogeneous NS0 cell lines differing in qMab were isolated from a pool of primary transfectants. The proteome of each stably transfected cell line was analyzed at mid-exponential growth phase by two-dimensional gel electrophoresis (2D-PAGE) and individual protein spot volume data derived from digitized gel images were compared statistically. To identify changes in protein abundance associated with qMab datasets were screened for proteins that exhibited either a linear correlation with cell line qMab or a conserved change in abundance specific only to the cell line with highest qMab. Several proteins with altered abundance were identified by mass spectrometry. Proteins exhibiting a significant increase in abundance with increasing qMab included molecular chaperones known to interact directly with nascent immunoglobulins during their folding and assembly (e.g., BiP, endoplasmin, protein disulfide isomerase). 2D-PAGE analysis showed that in all cell lines Mab light chain was more abundant than heavy chain, indicating that this is a likely prerequisite for efficient Mab production. In summary, these data reveal both the adaptive responses and molecular mechanisms enabling mammalian cells in culture to achieve high-level recombinant monoclonal antibody production.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/60/1/Smales_et_al_2004%2C_Biotechnology_%26_Bioengineering%2C_88.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>JOHN WILEY &amp; SONS</dc:publisher><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=15459912</dc:relation><dc:source>0006-3592</dc:source><dc:subject>Q</dc:subject><dc:title>Comparative proteomic analysis of GS-NSO murine myeloma cell lines with varying recombinant monoclonal antibody production rate</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Smales, C. Mark</rioxxterms:author><rioxxterms:author>Dinnis, Diane M.</rioxxterms:author><rioxxterms:author>Stansfield, Scott H.</rioxxterms:author><rioxxterms:author>Alete, Daniel E.</rioxxterms:author><rioxxterms:author>Sage, E.A.</rioxxterms:author><rioxxterms:author>Birch, John R.</rioxxterms:author><rioxxterms:author>Racher, Andrew J.</rioxxterms:author><rioxxterms:author>Marshall, Carol T.</rioxxterms:author><rioxxterms:author>James, David C.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1002/bit.20272</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:56
Date: 2017-07-17

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:56</identifier>
      <datestamp>2017-07-17T22:23:34Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The receptors for interleukin 2 (IL-2) and interleukin 15 (IL-15) in T cells share the IL-2R beta subunit (CD122) and gamma(C) subunit but have private alpha subunits. Despite utilizing the same receptor chains known to be necessary and sufficient to transduce IL-2 signals the two cytokines manifest different cellular effects. It is commonly held that the alpha subunit of the IL-2R (CD25) is involved solely in the generation of a high affinity receptor complex. This is questioned by the development of autoimmune diseases in instances where the expression of CD25 is absent. The timely expression of CD25 in the thymus has been linked with clonal deletion. Evidence from peripheral T cells indicates that survival signals arising from the intermediate affinity IL-2R (lacking CD25) do not require the activation of Janus kinase 3 (Jak3) but do require the presence of the membrane proximal region of the gamma(C) chain. This particular signalling pathway is not observed in the high affinity receptor complex where Jak3 is activated. Recent data point to CD25 having a surface distribution consistent with it being localized within membrane microdomains. Here we suggest that in the absence of CD25 expression, IL-2R activation occurs within the soluble membrane fraction. This membrane environment and the absence of CD25 promotes Jak3 independent signal transduction and induction of antiapoptotic mechanisms. T cell antigen receptor (TCR) signalling leads to the induction of CD25 expression, which localizes to membrane microdomains. There is a dynamic pre-association of CD25 and CD122 leading to the loose association of the heterodimer with membrane microdomains. High affinity IL-2R signalling in the context of CD25 and the microdomain environment is characterized by Jak3 activation. The relative levels of high to intermediate affinity receptor signalling determines whether a cell proliferates or undergoes activation induced cell death dependent upon cell status.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/56/1/Ellery_%26_Nicholls_2002_Immunology_%26_Cell_Biology_80_351-357.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Nature Publishing Group</dc:publisher><dc:source>0818-9641</dc:source><dc:subject>Q</dc:subject><dc:title>Possible mechanism for the alpha subunit of the interleukin-2 receptor (CD25) to influence interleukin-2 receptor signal transduction.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Ellery, Jonathan M.</rioxxterms:author><rioxxterms:author>Nicholls, Peter J.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2002</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:55
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:55</identifier>
      <datestamp>2016-04-27T14:11:36Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F33</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Cytokinesis in fission yeast involves the coordination of septum deposition with the contraction of a cytokinetic actomyosin ring. We have examined the role of the type V myosin Myo52 in the coupling of these two events by the construction of a series of deletion mutants of the Myo52 tail and a further mutant within the ATP binding domain of the head. Each mutant protein was ectopically expressed in fission yeast cells. Each truncation was assayed for the ability to localize to the cell poles and septum (the normal cellular locations of Myo52) and to rescue the morphology defects and temperature sensitivity of a myo52Delta strain. A region within the Myo52 tail (amino acids 1320-1503), with a high degree of similarity to the vesicle-binding domain of the budding yeast type V myosin Myo2p, was essential for Myo52's role in the maintenance of growth polarity and cell division. A separate region (amino acids 1180-1320) was required for Myo52 foci to move throughout the cytoplasm; however, constructs lacking this region, but which retained the ability to dimerize still associated with actin at sites of cell growth. Not all of the Myo52 truncations which localized rescued the morphological defects of myo52Delta, demonstrating that loss of function was not simply brought about by an inability of mutant proteins to target the correct cellular location. By contrast, Myo52 motor activity was required for both localization and cellular function. myo52Delta cells were unable to efficiently localize the beta-1,3-glucan synthase, Bgs1, either at the cell poles or at the division septum, regions of cell wall deposition. Bgs1 and Myo52 localized to vesicle-like dots at the poles in interphase and these moved together to the septum at division. These data have led to the formulation of a model in which Myo52 is responsible for the delivery of Bgs1 and associated molecules to polar cell growth regions during interphase. On the commencement of septum formation, Myo52 transports Bgs1 to the cell equator, thus ensuring the accurate deposition of beta-1,3-glucan at the leading edge of the primary septum.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/55/1/Mulvihill_et_al%2C_2006%2C_Cell_Motility_Cytoskeleton.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Wiley-Blackwell</dc:publisher><dc:source>0886-1544</dc:source><dc:subject>Q</dc:subject><dc:title>A critical role for the type V myosin, Myo52, in septum deposition and cell fission during cytokinesis in Schizosaccharomyces pombe.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Mulvihill, Daniel P.</rioxxterms:author><rioxxterms:author>Edwards, Suzanne R.</rioxxterms:author><rioxxterms:author>Hyams, Jeremy S.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2006</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1002/cm.20113</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:54
Date: 2017-06-28

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:54</identifier>
      <datestamp>2017-06-28T10:21:55Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F33</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>In dividing cells, the assembly and contraction of the cytokinetic actomyosin ring (CAR) is precisely coordinated with spindle formation and chromosome segregation. Despite having a cell wall, the fission yeast Schizosaccharomyces pombe forms a CAR reminiscent of the structure responsible for the cleavage of cells with flexible boundaries. We used the myo2-gc fission yeast strain in which the chromosomal copy of the type II myosin gene, myo2(+), is fused to the gene encoding green fluorescent protein (GFP) to investigate the dynamics of Myo2 recruitment to the cytokinetic actomyosin ring in living cells. Analysis of CAR formation in relation to spindle pole body (SPB) and centromere separation enabled us to pinpoint the timing of Myo2 recruitment into a stable CAR structure to the onset of anaphase A. Depolymerisation of actin with latrunculin B did not affect the timing of Myo2 accumulation at the cell equator (although Myo2 no longer formed a ring), whereas depolymerisation of microtubules with either thiabendazole (TBZ) or methyl 2-benzimidazolecarbamate (MBC) resulted in a delay of up to 90 minutes in CAR formation. Microtubule depolymerisation also delayed the localisation of other CAR components such as actin and Mid1/Dmf1. The delay of cytokinesis in response to loss of microtubule integrity was abolished in cells lacking the spindle assembly checkpoint protein Mad2 or containing non-functional Cdc16, a component of the fission yeast septation initiation network (SIN). The delay was also abolished in cells lacking Zfs1, a component of the previously described S. pombe cytokinesis checkpoint. Recruitment of the polo-related kinase, Plo1, a key regulator of CAR formation, to the SPBs was substantially reduced in TBZ in a Mad2-dependent manner. Loading of Cdc7, a component of the SIN and downstream of Plo1 in the cytokinesis pathway, onto the the SPBs was also delayed in TBZ to the same extent as CAR formation. We conclude that CAR formation is subject to regulation by the spindle assembly checkpoint via the loading of Plo1 onto the SPBs and the consequent activation of the SIN.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/54/1/Mulvihill_%26_Hyams_2002%2C_J_Cell_Science_115%2C_3575-3586.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Company of Biologists Ltd</dc:publisher><dc:source>0021-9533</dc:source><dc:subject>Q</dc:subject><dc:title>Cytokinetic actomyosin ring formation and septation in fission yeast are dependent on the full recruitment of the polo-like kinase Plo1 to the spindle pole body and a functional spindle assembly checkpoint.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Mulvihill, Daniel P.</rioxxterms:author><rioxxterms:author>Hyams, Jeremy S.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2002-09-15</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:53
Date: 2017-06-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:53</identifier>
      <datestamp>2017-06-27T21:18:28Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F33</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Myo2 truncations fused to green fluorescent protein (GFP) defined a C-terminal domain essential for the localization of Myo2 to the cytokinetic actin ring (CAR). The localization domain contained two predicted phosphorylation sites. Mutation of serine 1518 to alanine (S(1518)A) abolished Myo2 localization, whereas Myo2 with a glutamic acid at this position (S(1518)E) localized to the CAR. GFP-Myo2 formed rings in the septation initiation kinase (SIN) mutant cdc7-24 at 25 degrees C but not at 36 degrees C. GFP-Myo2S(1518)E rings persisted at 36 degrees C in cdc7-24 but not in another SIN kinase mutant, sid2-250. To further examine the relationship between Myo2 and the SIN pathway, the chromosomal copy of myo2(+) was fused to GFP (strain myo2-gc). Myo2 ring formation was abolished in the double mutants myo2-gc cdc7.24 and myo2-gc sid2-250 at the restrictive temperature. In contrast, activation of the SIN pathway in the double mutant myo2-gc cdc16-116 resulted in the formation of Myo2 rings which subsequently collapsed at 36 degrees C. We conclude that the SIN pathway that controls septation in fission yeast also regulates Myo2 ring formation and contraction. Cdc7 and Sid2 are involved in ring formation, in the case of Cdc7 by phosphorylation of a single serine residue in the Myo2 tail. Other kinases and/or phosphatases may control ring contraction.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/53/1/Mulvihill_et_al_2001%2C_Molecular_Biology_of_the_Cell_12%2C_4044-4053.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Society of Cell Biology</dc:publisher><dc:source>1059-1524 </dc:source><dc:subject>Q</dc:subject><dc:title>Localization of fission yeast type II myosin, Myo2, to the cytokinetic actin ring is regulated by phosphorylation of a C-terminal coiled-coil domain and requires a functional septation initiation network.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Mulvihill, Daniel P.</rioxxterms:author><rioxxterms:author>Barretto, Caroline</rioxxterms:author><rioxxterms:author>Hyams, Jeremy S.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001-12</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:52
Date: 2017-01-12

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is not a valid RIOXX record
PropertyError
rioxxterms:version_of_record'http://dx.doi.org/10.1016/S0960-9822(01)00322-0 ' is not a valid HTTP URI in rioxxterms:version_of_record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
rioxxterms:version_of_record'http://dx.doi.org/10.1016/S0960-9822(01)00322-0 ' is not a valid HTTP URI in rioxxterms:version_of_record
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:52</identifier>
      <datestamp>2017-01-12T22:55:09Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F33</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The class V myosins are actin-based motors that move a variety of cellular cargoes [1]. In budding yeast, their activity includes the relocation of a portion of the vacuole from the mother cell to the bud [2, 3]. Fission yeast cells contain numerous (approximately 80) small vacuoles. When S. pombe cells are placed in water, vacuoles fuse in response to osmotic stress [4]. Fission yeast possess two type V myosin genes, myo51(+) and myo52(+) [5]. In a myo51Delta strain, vacuoles were distributed throughout the cell, and mean vacuole diameter was identical to that seen in wild-type cells. When myo51Delta and wild-type cells were placed in water, vacuoles enlarged by fusion. In myo52Delta cells, by contrast, vacuoles were smaller and mostly clustered around the nucleus, and fusion in water was largely inhibited. When cells containing GFP-Myo52 were placed in water, Myo52 was seen to redistribute from the cell poles to the surface of the fusing vacuoles. Vacuole fusion in fission yeast was inhibited by the microtubule drug thiabendazole (TBZ) but not by the actin inhibitor latrunculin B. This is the first demonstration of the involvement of a type V myosin, possibly via an interaction with microtubules, in homotypic membrane fusion.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/52/1/Mulvihill_et_al_2001%2C_Current_Biology_11%2C_1124-1127.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Cell Press</dc:publisher><dc:source>0960-9822 </dc:source><dc:subject>Q</dc:subject><dc:title>Myosin V-mediated vacuole distribution and fusion in fission yeast.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Mulvihill, Daniel P.</rioxxterms:author><rioxxterms:author>Pollard, Patrick J.</rioxxterms:author><rioxxterms:author>Win, Thein Z.</rioxxterms:author><rioxxterms:author>Hyams, Jeremy S.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001-07-24</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1016/S0960-9822(01)00322-0 </rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:51
Date: 2017-01-16

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:51</identifier>
      <datestamp>2017-01-16T18:30:20Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Cryptococcus neoformans, a fungal pathogen of humans, causes fatal meningitis in immunocompromised patients. Its virulence is mainly determined by the elaboration of a polysaccharide capsule surrounding its cell wall. During its life, C. neoformans is confronted with and responds to dramatic variations in CO2 concentrations; one important morphological change triggered by the shift from its natural habitat (0.033% CO2) to infected hosts (5% CO2) is the induction of capsule biosynthesis. In cells, CO2 is hydrated to bicarbonate in a spontaneous reaction that is accelerated by carbonic anhydrases. Here we show that C. neoformans contains two beta-class carbonic anhydrases, Can1 and Can2. We further demonstrate that CAN2, but not CAN1, is abundantly expressed and essential for the growth of C. neoformans in its natural environment, where CO2 concentrations are limiting. Structural studies reveal that Can2 forms a homodimer in solution. Our data reveal Can2 to be the main carbonic anhydrase and suggest a physiological role for bicarbonate during C. neoformans growth. Bicarbonate directly activates the C. neoformans Cac1 adenylyl cyclase required for capsule synthesis. We show that this specific activation is optimal at physiological pH.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/51/1/Gewiss_Mogensen_et_al_2006%2C_Eukaryot_Cell%2C_5%2C_103-111.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Society for Microbiology</dc:publisher><dc:source>1535-9778</dc:source><dc:subject>Q</dc:subject><dc:title>Cryptococcus neoformans senses CO2 through the carbonic anhydrase Can2 and the adenylyl cyclase Cac1.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Gewiss Mogensen, Estelle</rioxxterms:author><rioxxterms:author>Janbon, Guihem</rioxxterms:author><rioxxterms:author>Chaloupka, James</rioxxterms:author><rioxxterms:author>Steegborn, Clemens</rioxxterms:author><rioxxterms:author>Fu, Man Shun</rioxxterms:author><rioxxterms:author>Moyrand, Frédérique</rioxxterms:author><rioxxterms:author>Klengel, Torsten</rioxxterms:author><rioxxterms:author>Pearson, David S.</rioxxterms:author><rioxxterms:author>Geeves, Michael A.</rioxxterms:author><rioxxterms:author>Buck, Jochen</rioxxterms:author><rioxxterms:author>Levin, Lonny R.</rioxxterms:author><rioxxterms:author>Mühlschlegel, Fritz A.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2006</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1128/EC.5.1.103-111.2006</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:50
Date: 2017-01-14

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:50</identifier>
      <datestamp>2017-01-14T14:25:39Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>In this study, we describe a simple method for the identification of Candida albicans in clinical samples. A total of 383 clinical isolates of Candida species were streaked onto chocolate agar and incubated for 48 h at 37 degrees C in the presence of an atmosphere of 6% CO2. All 208 of the C. albicans isolates tested, developed an easy to identify filamentous colony morphology. Of 175 other Candida species tested, 172 (98.3%) were distinguishable from C. albicans by their smooth colony morphology. Three isolates (1.7%) exhibited weak filamentation after prolonged incubation. Although not a routine medium in medical mycology a significant advantage of using chocolate agar lies in its use in clinical bacteriology laboratories for the isolation of fastidious bacteria. Implementation of the proposed method is applicable across a range of specimen types, thus allowing the direct identification of C. albicans in clinical samples. This simple method may allow a quicker entry into directed treatment.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/50/1/Sheth_et_al_2005%2C_Medical_Mycology_43%2C_735-738.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Oxford Journals</dc:publisher><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=16422305</dc:relation><dc:source>1232-986X</dc:source><dc:subject>Q</dc:subject><dc:title>Phenotypic identification of Candida albicans by growth on chocolate agar.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Sheth, Chirag C.</rioxxterms:author><rioxxterms:author>Johnson, Elizabeth</rioxxterms:author><rioxxterms:author>Baker, Mark E.</rioxxterms:author><rioxxterms:author>Haynes, Ken</rioxxterms:author><rioxxterms:author>Mühlschlegel, Fritz A.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2005</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1080/13693780500265998</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:48
Date: 2017-06-29

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:48</identifier>
      <datestamp>2017-06-29T04:49:11Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>In fungi, the cell wall plays a major role in host-pathogen interactions. Despite this, little is known about the molecular basis of cell wall assembly in Candida glabrata, which has emerged as the second most common cause of systemic candidosis. A C. glabrata gene family, CgGAS1-3, that shares significant homologies with both the GAS1 gene of Saccharomyces cerevisiae, which is necessary for cell wall assembly, and the pH-regulated genes PHR1 and PHR2 of Candida albicans, which are involved in cell wall assembly and required for virulence, has been cloned. Among the members of this family, CgGAS1-3 display a unique expression pattern. Both CgGAS1 and CgGAS2 are constitutively expressed. In contrast, CgGAS3 transcript was not detectable under any of the assayed conditions. The C. glabrata actin gene, CgACT1, has also been cloned to be used as a meaningful loading control in Northern blots. CgGAS1 and CgGAS2 were deleted by two different methodological approaches. A rapid PCR-based strategy by which gene disruption was achieved with short regions of homology (50 bp) was applied successfully to C. glabrata. DeltaCggas1 or DeltaCggas2 cells demonstrated similar aberrant morphologies, displaying an altered bud morphology and forming floccose aggregates. These phenotypes suggest a role for CgGAS1 and CgGAS2 in cell wall biosynthesis. Further evidence for this hypothesis was obtained by successful functional complementation of a gas1 null mutation in S. cerevisiae with the C. glabrata CgGAS1 or CgGAS2 gene.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/48/1/Weig_et_al_2001%2C_Microbiology_147_2007-2019.pdf</dc:identifier><dc:language>en</dc:language><dc:source>0002-4564</dc:source><dc:subject>Q</dc:subject><dc:title>A GAS-like gene family in the pathogenic fungus Candida glabrata.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Weig, Michael</rioxxterms:author><rioxxterms:author>Haynes, Ken</rioxxterms:author><rioxxterms:author>Rogers, Thomas R.</rioxxterms:author><rioxxterms:author>Kurzai, Oliver</rioxxterms:author><rioxxterms:author>Frosch, Matthias</rioxxterms:author><rioxxterms:author>Mühlschlegel, Fritz A.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:45
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:45</identifier>
      <datestamp>2016-04-27T13:47:37Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>One of the rate-limiting steps in protein folding has been shown to be the cis-trans isomerization of proline residues, which is catalyzed by a range of peptidylprolyl cis-trans isomerases. To characterize the interaction between model peptides and the periplasmic peptidylprolyl cis-trans isomerase SurA from E. coli, we employed a chemical cross-linking strategy that has been used previously to elucidate the interaction of substrates with other folding catalysts. The interaction between purified SurA and model peptides was significant in that it showed saturation and was abolished by denaturation of SurA; however the interaction was independent of the presence of proline residues in the model peptides. From results obtained by limited proteolysis we conclude that an N-terminal fragment of SurA, comprising 150 amino acids that do not contain the active sites involved in the peptidylprolyl cis-trans isomerization, is essential for the binding of peptides by SurA. This was confirmed by probing the interaction of the model peptide with the recombinant N-terminal fragment, expressed in Escherichia coli. Hence we propose that, similar to protein disulfide isomerase and other folding catalysts, SurA exhibits a modular architecture composed of a substrate binding domain and distinct catalytically active domains.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/45/1/Webb_et_al_2001%2C_J_Biol_Chem_276.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Society for Biochemistry and Molecular Biology</dc:publisher><dc:source>0021-9258</dc:source><dc:subject>Q</dc:subject><dc:title>Interaction of the periplasmic peptidylprolyl cis-trans isomerase SurA with model peptides. The N-terminal region of SurA id essential and sufficient for peptide binding.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Webb, Helen M.</rioxxterms:author><rioxxterms:author>Ruddock, Lloyd W.</rioxxterms:author><rioxxterms:author>Marchant, Rosalyn J.</rioxxterms:author><rioxxterms:author>Jonas, Kim</rioxxterms:author><rioxxterms:author>Klappa, Peter</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1074/jbc.M107508200</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:44
Date: 2017-06-28

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:44</identifier>
      <datestamp>2017-06-28T06:09:52Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Using a cross-linking approach, we have recently demonstrated that radiolabelled model peptides or misfolded proteins specifically interact in vitro with two members of the protein disulphide- isomerase family, namely PDI and PDIp, in a crude extract from sheep pancreas microsomes. In addition, we have shown that tyrosine and tryptophan residues within a peptide are the recognition motifs for the binding to PDIp. Here we examine non-peptide ligands and present evidence that a hydroxyaryl group is a structural motif for the binding to PDIp; simple constructs containing this group and certain xenobiotics and phytoestrogens, which contain an unmodified hydroxyaryl group, can all efficiently inhibit peptide binding to PDIp. To our knowledge this is the first time that the recognition motif of a molecular chaperone or folding catalyst has been specified as a simple chemical structure.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/44/1/Klappa_et_al_2001%2C_Biochem_J_354%2C_553-559.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Portland Press</dc:publisher><dc:source>0264-6021</dc:source><dc:subject>Q</dc:subject><dc:title>The pancreas-specific protein disulphide-isomerase PDIp interacts with a hydroxyaryl group in ligands.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Klappa, Peter</rioxxterms:author><rioxxterms:author>Freedman, Robert B.</rioxxterms:author><rioxxterms:author>Langenbuch, Martina</rioxxterms:author><rioxxterms:author>Lan, Michael S.</rioxxterms:author><rioxxterms:author>Robinson, Gary K.</rioxxterms:author><rioxxterms:author>Ruddock, Lloyd W.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1042/0264-6021:3540553</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:43
Date: 2017-06-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:43</identifier>
      <datestamp>2017-06-27T17:54:42Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/43/1/Wong_%26_Jeffries_2006%2C_Plant_Pathology_55%2C_331-342.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Wiley</dc:publisher><dc:source>0032-0862</dc:source><dc:subject>Q</dc:subject><dc:title>Diversity of pathogenic Fusarium populations associated with asparagus roots in decline soils in Spain and the UK.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Wong, J.Y.</rioxxterms:author><rioxxterms:author>Jeffries, Peter</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2006</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1111/j.1365-3059.2006.01360.x</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:42
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:42</identifier>
      <datestamp>2016-04-27T13:34:55Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The use of commercially available cryogenic vials (Microbank vials) stored at -70 degrees C for the storage and preservation of dermatophyte fungi was investigated. None of the 200 strains of dermatophytes examined, representing 21 species, showed a loss of viability after they had been stored for periods ranging from 1 week to 2 years at -70 degrees C. All strains showed typical colonial and microscopic morphologies following revival.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/42/1/Baker_%26_Jeffries_2006%2C_J_Clinical_Microbiology_44.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Society for Microbiology</dc:publisher><dc:source>0095-1137</dc:source><dc:subject>Q</dc:subject><dc:title>Use of commercially available cryogenic vials for long-term preservation of dermatophyte fungi.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Baker, M.</rioxxterms:author><rioxxterms:author>Jeffries, Peter</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2006</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1128/JCM.44.2.617-618.2006</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:41
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:41</identifier>
      <datestamp>2016-04-27T13:31:34Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/41/1/Kung%27u_%26_Jeffries_2001%2C_Ann_Appl_Biol_139.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Wiley</dc:publisher><dc:source>0003-4746</dc:source><dc:subject>Q</dc:subject><dc:title>Races and virulence of Fusarium oxysporum f.sp. cubense on local banana cultivars in Kenya.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Kung'u, Jackson N.</rioxxterms:author><rioxxterms:author>Jeffries, Peter</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1111/j.1744-7348.2001.tb00148.x</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:40
Date: 2017-06-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:40</identifier>
      <datestamp>2017-06-27T05:53:09Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Acacia mangium and Paraserianthes falcataria are leguminous tree species widely grown for timber in Indonesia and other tropical countries, yet little is known about the identity of their rhizobial symbionts. Polymerase chain reaction-restriction fragment length polymorphism-single-strand conformational polymorphism (PRS) analysis of the 16S rRNA gene was used along with sequencing to assess the diversity of 57 rhizobia isolated from nodules of A. mangium and P. falctaria in Indonesia. In total, 26 rhizobia isolated from A. mangium were analysed by PRS and sequencing. The PRS patterns indicated that 12 (46%) clustered with Bradyrhizobium elkanii, 13 (50%) with B. lianoningense/japonicum and one (4%) with Mesorhizobium loti. Thirty-one isolates were analysed from P. falcataria: five (16%) clustered with B. elkanii and 26 (84%) with B. lianoningense/japonicum. These results were confirmed by phylogenetic analysis of sequences. Intraspecific diversity of the 16S rRNA genes from rhizobia nodulating A. mangium and P. falcataria revealed by PRS was low, only one genotype was found within the isolates that clustered with B. elkanii and two within the B. liaoningense/japonicum group. These Bradyrhizobium species are apparently ubiquitous throughout the Indonesian archipelago and it is clear why the two tree species are able to successfully establish outside their native range without the need for inoculation with indigenous rhizobia.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/40/1/Clapp_et_al_2001%2C_Env_Micro_3%2C_273-280.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Wiley</dc:publisher><dc:source>1462-2912</dc:source><dc:subject>Q</dc:subject><dc:title>Ribotyping of rhizobia nodulating Acacia mangium and Paraserianthes falcataria from different geographical areas in Indonesia using PCR-RFLP-SSCP (PRS) and sequencing.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Clapp, Justin P.</rioxxterms:author><rioxxterms:author>Mansur, Irdika</rioxxterms:author><rioxxterms:author>Dodd, John C.</rioxxterms:author><rioxxterms:author>Jeffries, Peter</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1046/j.1462-2920.2001.00191.x</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:39
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:39</identifier>
      <datestamp>2016-04-27T13:19:16Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/39/1/Cragg%2C_Hertzler_%26_Howard_2004%2C_Chem_Commun.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Royal Society of Chemistry</dc:publisher><dc:source>1359-7345</dc:source><dc:subject>Q</dc:subject><dc:title>In flagrante metallo-cyclophane self-assembly?</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Cragg, P.J.</rioxxterms:author><rioxxterms:author>Heirtzler, Fenton R.</rioxxterms:author><rioxxterms:author>Howard, Mark J.</rioxxterms:author><rioxxterms:author>Prokes, I.</rioxxterms:author><rioxxterms:author>Weyhermuller, T.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:37
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:37</identifier>
      <datestamp>2016-04-27T13:09:25Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The UBX domain is an 80 amino acid residue module that is present typically at the carboxyl terminus of a variety of eukaryotic proteins. In an effort to elucidate the function of UBX domains, we solved the three-dimensional structure of the UBX domain of human Fas-associated factor-1 (FAF1) by NMR spectroscopy. The structure has a beta-Grasp fold characterised by a beta-beta-alpha-beta-beta-alpha-beta secondary-structure organisation. The five beta strands are arranged into a mixed sheet in the order 21534. The longer first helix packs across the first three strands of the sheet, and a second shorter 3(10) helix is located in an extended loop connecting strands 4 and 5. In the absence of significant sequence similarity, the UBX domain can be superimposed with ubiquitin with an r.m.s.d. of 1.9 A, suggesting that the two structures share the same superfold, and an evolutionary relationship. However, the absence of a carboxyl-terminal extension containing a double glycine motif and of suitably positioned lysine side-chains makes it highly unlikely that UBX domains are either conjugated to other proteins or part of mixed UBX-ubiquitin chains. Database searches revealed that most UBX domain-containing proteins belong to one of four evolutionarily conserved families represented by the human FAF1, p47, Y33K, and Rep8 proteins. A role of the UBX domain in ubiquitin-related processes is suggested.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/37/1/Buchberger_et_al_2001%2C_J_Mol_Biol.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Elsevier</dc:publisher><dc:source>0022-2836</dc:source><dc:subject>Q</dc:subject><dc:title>The UBX domain: a widespread ubiquitin-like module.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Buchberger, Alexander</rioxxterms:author><rioxxterms:author>Howard, Mark J.</rioxxterms:author><rioxxterms:author>Proctor, Mark</rioxxterms:author><rioxxterms:author>Bycroft, Mark</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1006/jmbi.2001.4462</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:36
Date: 2016-11-15

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:36</identifier>
      <datestamp>2016-11-15T12:02:02Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The co-solvent 2,2,2-trifluoroethanol (TFE) has been often used to aid formation of secondary structure in solution peptides or alternately as a denaturant within protein folding studies. Hen egg white lysozyme (HEWL) and a synthetic model peptide defining HEWL helix-4 were used as comparative model systems to systematically investigate the effect of increasing TFE concentrations on the structure of proteins and peptides. HEWL was analyzed using NMR, far-UV CD and fluorescence spectroscopy; with correlation of these results towards changes in enzymatic activity and the helix-4 peptide was analysed using NMR. Data illustrates two conflicting modes of interaction: Low TFE concentrations stabilize tertiary structure, observed from an increase in the number of NMR NOE contacts. Higher TFE concentrations denatured HEWL with the loss of lysozyme tertiary structure. The effects of TFE upon secondary structural elements within HEWL are distinct from those observed for the helix-4 peptide. This illustrates a dissimilar interaction of TFE towards both protein and peptide at equivalent TFE concentrations. The concentration that TFE promotes stabilization over denaturation is likely to be protein dependent although the structural action can be extrapolated to other protein systems with implications for the use of TFE in structural stability studies.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/36/1/Povey_et_al_2006_J_Structural_Biology_in_press.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Academic Press Inc</dc:publisher><dc:source>1047-8477</dc:source><dc:subject>Q</dc:subject><dc:title>Comparison of the effects of 2,2,2-trifluoroethanol on peptide and protein structure and function.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Povey, Jane F.</rioxxterms:author><rioxxterms:author>Smales, C. Mark</rioxxterms:author><rioxxterms:author>Hassard, S.J.</rioxxterms:author><rioxxterms:author>Howard, Mark J.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2007</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1016/j.jsb.2006.07.008</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:32
Date: 2017-01-15

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:32</identifier>
      <datestamp>2017-01-15T20:38:38Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>7375626A656374733D52:5243:524330323534</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The ERBB3 gene is expressed as a 6.2- and a 1.4-kb transcript. The former encodes the full-length transmembrane protein and the latter a truncated extracellular fragment consisting of 140 amino acids of the c-erbB-3 protein followed by 43 unique residues. We have examined the expression of the two ERBB3 transcripts by Northern blotting in cancer cell lines and normal human fetal and adult tissues. We expressed the truncated receptor fragment and showed that it was glycosylated, probably with a single N-linked complex sugar chain, and that the protein was a 58-kDa disulphide-linked dimer. We were able to crosslink iodinated neuregulin (NRG)-1beta to the full-length solubilised receptor but not to the truncated dimeric protein. Using Western blot analysis, the truncated protein was shown to be present in cell lysates and, using immunoelectron microscopy, in vesicular structures within cells and associated with the plasma cell membrane.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/32/1/Srinivasan_et_al_2001%2C_Cell_Signalling_13%2C_321-330.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Elsevier Science B.V., Amsterdam.</dc:publisher><dc:source>0898-6568</dc:source><dc:subject>Q</dc:subject><dc:subject>RC0254</dc:subject><dc:title>Intracellular expression of the truncated extracellular domain of c-erbB-3/HER3.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Srinivasan, Radhika</rioxxterms:author><rioxxterms:author>Leverton, Kate E.</rioxxterms:author><rioxxterms:author>Sheldon, Helen</rioxxterms:author><rioxxterms:author>Hurst, Helen C.</rioxxterms:author><rioxxterms:author>Sarraf, Catherine</rioxxterms:author><rioxxterms:author>Gullick, William J.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1016/S0898-6568(01)00155-3</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:29
Date: 2017-06-28

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:29</identifier>
      <datestamp>2017-06-28T02:27:03Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F33</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Several determinants of aging, including metabolic capacity and genetic stability, are recognized in both yeast and humans. However, many aspects of the pathways leading to cell death remain to be elucidated. Here we report a role for the actin cytoskeleton both in cell death and in promoting longevity. We have analyzed yeast strains expressing mutants with either increased or decreased actin dynamics. We show that decreased actin dynamics causes depolarization of the mitochondrial membrane and an increase in reactive oxygen species (ROS) production, resulting in cell death. Important, however, is the demonstration that increasing actin dynamics, either by a specific actin allele or by deletion of a gene encoding the actin-bundling protein Scp1p, can increase lifespan by over 65%. Increased longevity appears to be due to these cells producing lower than wild-type levels of ROS. Homology between Scp1p and mammalian SM22/transgelin, which itself has been isolated in senescence screens, suggests a conserved mechanism linking aging to actin stability.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/29/1/Gourlay_et_al_2004%2C_J_Cell_Biol_164%2C_803-809.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Rockefeller University Press</dc:publisher><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=15024029</dc:relation><dc:source>0021-9525 </dc:source><dc:subject>Q</dc:subject><dc:title>A role for the actin cytoskeleton in cell death and aging in yeast.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Gourlay, Campbell W.</rioxxterms:author><rioxxterms:author>Carpp, Lindsay N.</rioxxterms:author><rioxxterms:author>Timpson, Paul</rioxxterms:author><rioxxterms:author>Winder, Steven J.</rioxxterms:author><rioxxterms:author>Ayscough, Kathryn R.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1083/jcb.200310148</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:28
Date: 2017-01-14

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:28</identifier>
      <datestamp>2017-01-14T03:50:22Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F33</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The build up of reactive oxygen species (ROS) is known to contribute to a reduction in the lifespan of a cell and to their degeneration in diseases such as Alzheimer's and tissue ischaemia. It is therefore important to elucidate pathways that regulate cellular oxidative stress. We have previously shown that actin dynamics can affect the oxidative-stress burden on a yeast cell and thereby its potential lifespan. To elucidate further the connection between actin dynamics and oxidative stress, we sought to identify regulators of this process. The actin regulatory proteins Sla1p and End3p are important in maintaining a rapid turnover of F-actin in cortical patches. We show that cells expressing a mutated form of Sla1p or lacking End3p display markers of apoptosis such as depolarized mitochondrial membranes and elevated levels of reactive oxygen species. Overexpression of the ubiquitin ligase RSP5 can alleviate the oxidative-stress phenotype observed in cells lacking End3p by targeting Sla1p to the cortex and restoring actin remodelling capability. We also demonstrate that overexpression of PDE2, a negative regulator of the Ras/cAMP pathway rescues actin dynamics, reduces oxidative stress sensitivity and restores viability in deltaend3 cells. Our data suggest, for the first time, that a physiological link exists between actin regulation and cAMP signalling that regulates apoptosis in yeast.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/28/1/Gourlay_%26_Ayscough_2005%2C_J_Cell_Sci_118%2C_2119-2132.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Company of Biologists</dc:publisher><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=15855235</dc:relation><dc:source>0021-9533 </dc:source><dc:subject>Q</dc:subject><dc:title>Identification of an upstream regulatory pathway controlling actin-mediated apoptosis in yeast</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Gourlay, Campbell W.</rioxxterms:author><rioxxterms:author>Ayscough, Kathryn R.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2005</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1242/jcs.02337</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:27
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:27</identifier>
      <datestamp>2016-04-27T11:45:37Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Diaphanous-related formins (Drf) are activated by Rho GTP binding proteins and induce polymerization of unbranched actin filaments. They contain three formin homology domains. Evidence as to the effect of formins on actin polymerization were obtained using FH2/FH1 constructs of various length from different Drfs. Here we define the core FH2 domain as a proteolytically stable domain of approximately 338 residues. The monomeric FH2 domains from mDia1 and mDia3 inhibit polymerization of actin and can bind in a 1:1 complex with F-actin at micromolar concentrations. The X-ray structure analysis of the domain shows an elongated, crescent-shaped molecule consisting of three helical subdomains. The most highly conserved regions of the domain span a distance of 75 A and are both required for barbed-end inhibition. A construct containing an additional 72 residue linker has dramatically different properties: It oligomerizes and induces actin polymerization at subnanomolar concentration.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/27/1/Shimada_et_al_2004_Mol_Cell.pdf</dc:identifier><dc:language>en</dc:language><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=14992721</dc:relation><dc:source>1097-2765</dc:source><dc:subject>Q</dc:subject><dc:title>The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Shimada, Atsushi</rioxxterms:author><rioxxterms:author>Nyitrai, Miklos</rioxxterms:author><rioxxterms:author>Vetter, Ingrid R.</rioxxterms:author><rioxxterms:author>Kuhlmann, Dorothee</rioxxterms:author><rioxxterms:author>Bugyi, Beata</rioxxterms:author><rioxxterms:author>Narumiya, Shuh</rioxxterms:author><rioxxterms:author>Geeves, Michael A.</rioxxterms:author><rioxxterms:author>Wittinghofer, Alfred</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1016/S1097-2765(04)00059-0</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:26
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:26</identifier>
      <datestamp>2016-04-27T11:42:09Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Competing models for the coordination of processive stepping in kinesin can be tested by introducing a roadblock to prevent lead head attachment. We used T93N, an irreversibly binding mutant monomer, as a roadblock, and measured the rates of nucleotide-induced detachment of kinesin monomers or dimers with and without the T93N roadblock using microflash photolysis combined with stopped flow. Control nucleotide-induced monomer (rK340) unbinding was 73.6 s(-1) for ATP and 40.5 s(-1) for ADP. Control ADP-induced dimer (rK430) unbinding was 18.6 s(-1). Added 20 mM Pi slowed both monomer and dimer unbinding. With the roadblock in place, lead head attachment of dimers is prevented and ATP-induced trail head unbinding was then 42 s(-1). This is less than two-fold slower than the stepping rate of unimpeded rK430 dimers (50-70 s(-1)), indicating that during walking, lead head attachment induces at most only a slight (less than two-fold) acceleration of trail head detachment. As we discuss, this implies a coordination model having very fast (&gt;2000 s(-1)) ATP-induced attachment of the lead head, followed by slower, strain-sensitive ADP release from the lead head.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/26/1/Crevel_et_al_2004_EMBO_J.pdf</dc:identifier><dc:language>en</dc:language><dc:source>0261-4189</dc:source><dc:subject>Q</dc:subject><dc:title>What kinesin does at roadblocks: the coordination mechanism for molecular walking.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Crevel, Isabelle M‐TC</rioxxterms:author><rioxxterms:author>Nyitrai, Miklos</rioxxterms:author><rioxxterms:author>Alonso, Maria C.</rioxxterms:author><rioxxterms:author>Weiss, Stefan</rioxxterms:author><rioxxterms:author>Geeves, Michael A.</rioxxterms:author><rioxxterms:author>Cross, Robert A.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1038/sj.emboj.7600042</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:24
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:24</identifier>
      <datestamp>2016-04-27T11:37:28Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F31</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Light chain phosphorylation is the key event that regulates smooth and non-muscle myosin II ATPase activity. Here we show that both heads of smooth muscle heavy meromyosin (HMM) bind tightly to actin in the absence of nucleotide, irrespective of the state of light chain phosphorylation. In striking contrast, only one of the two heads of unphosphorylated HMM binds to actin in the presence of ADP, and the heads have different affinities for ADP. This asymmetry suggests that phosphorylation alters the mechanical coupling between the heads of HMM. A model that incorporates strain between the two heads is proposed to explain the data, which have implications for how one head of a motor protein can gate the response of the other.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/24/1/Berger_et_al_2001%2C_J_Biol_Chem.pdf</dc:identifier><dc:language>en</dc:language><dc:source>0021-9258</dc:source><dc:subject>Q</dc:subject><dc:title>ADP binding induces an asymmetry between the heads of unphosphorylated myosin.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Berger, C.E.M.</rioxxterms:author><rioxxterms:author>Fagnant, P.M.</rioxxterms:author><rioxxterms:author>Heizmann, S.</rioxxterms:author><rioxxterms:author>Trybus, K.M.</rioxxterms:author><rioxxterms:author>Geeves, Michael A.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1074/jcb.M100524200</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:23
Date: 2017-06-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:23</identifier>
      <datestamp>2017-06-27T18:08:21Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>De novo material synthesis and application. Novel material with unique properties produced for  Philips Medical Systems Division and others  as part of international collaboration on drug delivery, development of SELDI-ToF and contrasting of patients in medical imaging . Competitively funded with EU FP6 IP project grant 12,000,000 Euro.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/23/1/Sen_et_al_2006_JACS_7130-7131.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Chemical Society</dc:publisher><dc:relation>http://dx.doi.org/10.1021/ja061393q</dc:relation><dc:source>0002-7863 </dc:source><dc:subject>Q</dc:subject><dc:title>Mesoporous silica-magnetite nanocomposite: fabrication and applications in magnetic bioseparations.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Sen, Tapas</rioxxterms:author><rioxxterms:author>Sebastianelli, Antonio</rioxxterms:author><rioxxterms:author>Bruce, Ian J.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2006</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1021/ja061393q</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:22
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:22</identifier>
      <datestamp>2016-04-27T11:29:11Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>A versatile and inexpensive method for the introduction of amine groups onto the surface of silica-coated magnetite composite nanoparticles has been established based on the condensation of (aminopropyl)triethoxysilane (APTS). The process was observed to be sensitive to a range of variables, and a range of silane surface-modified nanoparticles was synthesized under various reaction conditions, that is, solvent systems [water, tetrahydrofuran (THF), ethanol, or 1:1 mixtures of them], reaction times (from 1 to 24 h), and temperatures (18, 50, and 70 degrees C), with water as the catalyst and silane at either 0.2% or 2% (w/v) in an attempt to optimize the process. The products of the various reactions were characterized in terms of their possession of surface -NH2 groups, morphologies, and properties with respect to DNA binding and elution before being modified with a single-stranded oligonucleotide capture sequence. It was observed that careful manipulation of temperature, time, and solvent conditions was important for optimal silanization of the nanoparticles, and in our experiments best results were obtained when silanization of the particles in suspension involved use of water as the solvent and APTS at 0.2% (w/v) and when the reaction was conducted at room temperature for 5 h and was preceded by ultrasonication of the particle suspension. The materials produced were used in experiments to selectively capture complementary nucleic acid sequences by hybridization after grafting with an oligonucleotide. The efficiency of the oligonucleotide-modified particles in the capture experiments was observed to be directly related to the original density of amine groups present at the surface of the support. The results indicate that surface engineering of the nanoparticles was possible by silanization under defined, optimized conditions. This approach could be extended to the activation of such surfaces and other materials with other functional groups.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/22/1/Bruce_%26_Sen_2005_Langmuir.pdf</dc:identifier><dc:language>en</dc:language><dc:relation>http://dx.doi.org/10.1021/la050553t</dc:relation><dc:source>0743-7463 </dc:source><dc:subject>Q</dc:subject><dc:title>Surface modification of magnetic nanoparticles with alkoxysilanes and their application in magnetic bioseparations.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Bruce, Ian J.</rioxxterms:author><rioxxterms:author>Sen, Tapas</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2005-07</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1021/la050553t</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:21
Date: 2017-06-29

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:21</identifier>
      <datestamp>2017-06-29T04:52:57Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Magnetically responsive COOH-polydicarbazole-magnetite nanocomposites have been prepared by chemical oxidation of three COOH-dicarbazole monomers and - in the presence of magnetite nanoparticles. These functionalized nanoparticles have been tested for DNA hybridization experiments.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/21/1/Lellouche_et_al_2004%2C_Chemical_Communications%2C_560-561.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Royal Society of Chemistry</dc:publisher><dc:relation>http://dx.doi.org/doi:10.1039/b309375a</dc:relation><dc:source>1359-7345 </dc:source><dc:subject>Q</dc:subject><dc:title>New magnetically responsive polydicarbazole-magnetite nanoparticles.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Lellouche, Jean-Paul</rioxxterms:author><rioxxterms:author>Perlman, Nurit</rioxxterms:author><rioxxterms:author>Joseph, Augustine</rioxxterms:author><rioxxterms:author>Govindaraji, Senthil</rioxxterms:author><rioxxterms:author>Buzhansky, Ludmila</rioxxterms:author><rioxxterms:author>Yakir, Aline</rioxxterms:author><rioxxterms:author>Bruce, Ian J.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004-03</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1039/b309375a</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:20
Date: 2017-06-29

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:20</identifier>
      <datestamp>2017-06-29T04:48:03Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The complete genetic characterisation of Tn5530 in Burkholderia cepacia strain 2a (pIJB1) has been accomplished, indicating that it is a Tn3-like transposon with a complex structure bearing operons for the catabolism of 2,4-dichlorophenoxyacetate (2,4-D) and malonate. Tn5530 is terminated at both ends by the IS1071::IS1471 element and the 2,4-D- and malonate-dissimilatory operons are separated by a region encoding a putA and lrp gene and a gene encoding a chloride channel protein. The chloride channel protein may have a role in the expulsion of chloride ions liberated by the dissimilation of 2,4-D. In addition, a putative transposase with a high level of sequence similarity to those of plasmid pGH1 from Xanthomonas campestris pv. vesicatoria and Pseudomonas syringae pv. glycinea, and a transcription factor similar to those of the TetR family with low but significant levels of sequence similarity to those identified in a number of other organisms was observed. The entire Tn5530 sequence length, including the IS1071::IS1471 elements, was found to be 40,956bp, and pIJB1 was replicon-typed and otherwise characterised as being of the IncP-1beta subgroup, bearing merA and merD genes conferring resistance to mercuric chloride. The rate of uptake of 2,4-D by B. cepacia strain 2a was observed to proceed more readily at acid pH, suggesting involvement of the undissociated form of the compound. Uptake did not show saturation kinetics, was concentration-dependent, and appeared to occur in two stages; an initial accumulation followed by a linear second phase. Uptake could be inhibited by sodium azide but not by arsenate, N,N(')-dicyclohexylcarbodi-imide (DCCD) or carbonylcyanide m-chlorophenyl-hydrazone (CCCP) suggesting that it is not energy-dependent.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/20/1/Poh_et_al_2002%2C_Plasmid_48%2C_1-12.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Elsevier</dc:publisher><dc:source>0147-619X</dc:source><dc:subject>Q</dc:subject><dc:title>Complete characterisation of Tn5530 from Burkholderia cepacia strain 2a (pIJB1) and studies of 2,4-dichlorophenoxyacetate uptake by the organism.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Poh, R.P.-C.</rioxxterms:author><rioxxterms:author>Smith, A.R.W.</rioxxterms:author><rioxxterms:author>Bruce, Ian J.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2002</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:19
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:19</identifier>
      <datestamp>2016-04-27T10:46:04Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Bacterial-host attachment by means of bacterial adhesins is a key step in host colonization. Phase variation (reversible on-off switching) of the type 1 fimbrial adhesin of Escherichia coli involves a DNA inversion catalyzed by FimB (switching in either direction) or FimE (mainly on-to-off switching). fimB is separated from the divergent yjhATS operon by a large (1.4 kbp) intergenic region. Short ( approximately 28 bp) cis-active elements (regions 1 and 2) close to yjhA stimulate fimB expression and are required for sialic acid (Neu(5)Ac) sensitivity of its expression [El-Labany, S., Sohanpal, B. K., Lahooti, M., Akerman, R. &amp; Blomfield, I. C. (2003) Mol. Microbiol. 49, 1109-1118]. Here, we show that whereas NanR, a sialic acid-response regulator, binds to region 1, NagC, a GlcNAc-6P-responsive protein, binds to region 2 instead. The NanR- and NagC-binding sites lie adjacent to deoxyadenosine methylase (Dam) methylation sites (5'-GATC) that are protected from modification, and the two regulators are shown to be required for methylation protection at regions 1 and 2, respectively. Mutations in nanR and nagC diminish fimB expression, and both fimB expression and FimB recombination are inhibited by GlcNAc (3- and &gt;35-fold, respectively). Sialic acid catabolism generates GlcNAc-6-P, and whereas GlcNAc disrupts methylation protection by NagC alone, Neu(5)Ac inhibits the protection mediated by both NanR and NagC as expected. Type 1 fimbriae are proinflammatory, and host defenses enhance the release of both Neu(5)Ac and GlcNAc by a variety of mechanisms. Inhibition of type 1 fimbriation by these amino sugars may thus help balance the interaction between E. coli and its hosts.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/19/1/Sohanpal_et_al_2004%2C_PNAS.pdf</dc:identifier><dc:language>en</dc:language><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=15534208</dc:relation><dc:source>Proceedings of the National Academy of Sciences of the United States of America</dc:source><dc:subject>Q</dc:subject><dc:title>Integrated regulatory responses of fimB to N-acetylneuraminic (sialic) acid and GlcNAc in Escherichia coli K-12.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Sohanpal, Baljinder K.</rioxxterms:author><rioxxterms:author>El-Labany, Sammia</rioxxterms:author><rioxxterms:author>Lahooti, Maryam</rioxxterms:author><rioxxterms:author>Plumbridge, Jacqueline A.</rioxxterms:author><rioxxterms:author>Blomfield, Ian C.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1073/pnas.0405821101</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:18
Date: 2017-01-17

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:18</identifier>
      <datestamp>2017-01-17T03:02:22Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>The phase variation of type 1 fimbriation in Escherichia coli is controlled by the inversion of a 314 bp element of DNA, determined by FimB (switching in both directions) or FimE (switching from the ON-to-OFF orientation predominantly), and influenced by auxiliary factors IHF, Lrp and H-NS. The fimB gene is separated from the divergently transcribed yjhATS operon by a large (1.4 kbp) intergenic region of unknown function. Here, we show that fimB expression is regulated by multiple cis-active sequences that lie far upstream (&gt;600 bp) of the transcription start sites for the recombinase gene. Two regions characterized further (regions 1 and 2) show sequence identity, and each coincides with a methylation-protected Dam (5'-GATC) site. Regions 1 and 2 apparently control fimB expression by an antirepression mechanism that involves additional sequences proximal to yjhA. Region 1 encompasses a 27 bp DNA sequence conserved upstream of genes known (nanAT ) or suspected (yjhBC) to be involved in sialic acid metabolism, and we show that FimB expression and recombination are suppressed by N-acetylneuraminic acid. We propose that E. coli recognizes the amino sugars as a harbinger of potential host defence activation, and suppresses the expression of type 1 fimbriae in response.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/18/1/El-Labany_et_al_2003%2C_Mol_Microbiol.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Wiley</dc:publisher><dc:relation>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=12890032</dc:relation><dc:source>0950-382X</dc:source><dc:subject>Q</dc:subject><dc:title>Distant cis-active sequences and sialic acid control the expression of fimB in Escherichia coli K-12.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>El-Labany, Sammia</rioxxterms:author><rioxxterms:author>Sohanpal, Baljinder K.</rioxxterms:author><rioxxterms:author>Lahooti, Maryam</rioxxterms:author><rioxxterms:author>Akerman, Robert</rioxxterms:author><rioxxterms:author>Blomfield, Ian C.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2003</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1046/j.1365-2958.2003.03624.x</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:17
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:17</identifier>
      <datestamp>2016-04-27T10:32:50Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Phase variation of type 1 fimbriae of Escherichia coli requires the site-specific recombination of a short invertible element. Inversion is catalyzed by FimB (switching in either direction) or FimE (inversion mainly from on to off) and is influenced by auxiliary factors integration host factor (IHF) and leucine-responsive regulatory protein (Lrp). These proteins bind to sites (IHF site II and Lrp sites 1 and 2) within the invertible element to stimulate recombination, presumably by bending the DNA to enhance synapses. Interaction of Lrp with a third site (site 3) cooperatively with sites 1 and 2 (termed complex 1) impedes recombination. Inversion is stimulated by the branched-chain amino acids (particularly leucine) and alanine, and according to a current model, the amino acids promote the selective loss of Lrp from site 3 (complex 2). Here we show that the central portion of the fim invertible element, situated between Lrp site 3 and IHF site II, is dispensable for FimB recombination but that this region is also required for full amino acid stimulation of inversion. Further work reveals that the region is likely to contain multiple regulatory elements. Lrp site 3 is shown to bind the regulatory protein with low affinity, and a mutation that enhances binding to this element is found both to diminish the stimulatory effects of IVLA on FimB recombination and to inhibit recombination in the absence of the amino acids. The results obtained emphasize the importance of Lrp site 3 as a control element but also highlight the complexity of the regulatory system that affects this site.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/17/1/Lahooti%2C_Roesch%2C_Blomfield_2005%2C_J_Bacteriol.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>American Society for Microbiology</dc:publisher><dc:source>0021-9193</dc:source><dc:subject>Q</dc:subject><dc:title>Modulation of the sensitivity of FimB recombination to branched-chain amino acids and alanine in Escherichia coli K-12.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Lahooti, Maryam</rioxxterms:author><rioxxterms:author>Roesch, Paula L.</rioxxterms:author><rioxxterms:author>Blomfield, Ian C.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2005</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1128/JB.187.18.6273-6280.2005</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:16
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:16</identifier>
      <datestamp>2016-04-27T10:28:37Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F32</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>Phase-variable expression of type 1 fimbriae is, in part, controlled by site-specific DNA inversion of the fim switch in Escherichia coli. Of the two fim recombinases (FimB and FimE) that catalyse the inversion reaction, FimE exhibits a strong bias for phase switching from the ON to the OFF orientation. The specificity associated with fimE is the result of two different mechanisms: (i) FimE exhibits a preference for the invertible element in the ON orientation as substrate for recombination; (ii) the invertible element in the OFF orientation acts in cis to inhibit recombinase activity (orientational control). We show here that the invertible element negatively regulates fimE, even though expression of a fimE-lacZYA transcriptional fusion is unaffected by orientational control. The fimE transcript extends into the invertible region and hence switch ON-specific and switch OFF-specific mRNA contain different sequences. Furthermore, we show that orientational control is suppressed by the insertion of a structured RNA (tRNA(Gly)) between fimE and the fim switch, indicating that the switch OFF-specific mRNA is inactivated by 3' to 5' degradation. Analysis of the fim switch reveals that it contains two inhibitory elements that exert orientational control independently.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/16/1/Sohanpal_et_al_2001_Mol_Microbiol.pdf</dc:identifier><dc:language>en</dc:language><dc:source>0950-382X</dc:source><dc:subject>Q</dc:subject><dc:title>Orientational control of fimE expression in Escherichia coli.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Sohanpal, Baljinder K.</rioxxterms:author><rioxxterms:author>Kulasekara, Hemantha Don</rioxxterms:author><rioxxterms:author>Bonnen, Alice</rioxxterms:author><rioxxterms:author>Blomfield, Ian C.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2001</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version><rioxxterms:version_of_record>http://dx.doi.org/10.1046/j.1365-2958.2001.02655.x</rioxxterms:version_of_record></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:15
Date: 2016-04-27

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:15</identifier>
      <datestamp>2016-04-27T10:20:22Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D51:5131</setSpec>
      <setSpec>74797065733D61727469636C65</setSpec>
      <setSpec>6469766973696F6E733D466163756C74696573:3132303030:3132313030:31323130302F33</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><dc:description>We define here a previously unrecognized structural element close to the heart muscle plasma membrane at the intercalated disc where the myofibrils lead into the adherens junction. At this location, the plasma membrane is extensively folded. Immunofluorescence and immunogold electron microscopy reveal a spectrin-rich domain at the apex of the folds. These domains occur at the axial level of what would be the final Z-disc of the terminal sarcomere in the myofibril, although there is no Z-disc-like structure there. However, a sharp transitional boundary lies between the myofibrillar I-band and intercalated disc thin filaments, identifiable by the presence of Z-disc proteins, alpha-actinin, and N-terminal titin. This allows for the usual elastic positioning of the A-band in the final sarcomere, whereas the transduction of the contractile force normally associated with the Z-disc is transferred to the adherens junctions at the plasma membrane. The axial conjunction of the transitional junction with the spectrin-rich domains suggests a mechanism for direct communication between intercalated disc and contractile apparatus. In particular, it provides a means for sarcomeres to be added to the ends of the cells during growth. This is of particular relevance to understanding myocyte elongation in dilated cardiomyopathy.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/15/1/Bennett_et_al_2006%2C_Mol_Biol_Cell.pdf</dc:identifier><dc:language>en</dc:language><dc:source>1059-1524</dc:source><dc:subject>Q1</dc:subject><dc:title>The transitional junction: a new functional subcellular domain at the intercalated disc.</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Bennett, Pauline M.</rioxxterms:author><rioxxterms:author>Maggs, Alison M.</rioxxterms:author><rioxxterms:author>Baines, Anthony J.</rioxxterms:author><rioxxterms:author>Pinder, Jennifer C.</rioxxterms:author><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2006</rioxxterms:publication_date><rioxxterms:type>Journal Article/Review</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>
ID: oai:kar.kent.ac.uk:4
Date: 2016-06-07

RIOXX

Base RIOXX scheme designed for low-level interoperability
This is a valid RIOXX record

RCUK-RIOXX

RCUK RIOXX scheme for reporting of open access publications funded through UK Research Council grants
This is not a valid RCUK-RIOXX record
PropertyError
dcterms:dateAcceptedMinimum of 1 value(s) required for dcterms:dateAccepted - found 0 values
ali:license_refMinimum of 1 value(s) required for ali:license_ref - found 0 values
<record>
    <header>
      <identifier>oai:kar.kent.ac.uk:4</identifier>
      <datestamp>2016-06-07T15:37:55Z</datestamp>
      <setSpec>7374617475733D707562</setSpec>
      <setSpec>7375626A656374733D5A:5A41</setSpec>
      <setSpec>7375626A656374733D5A:5A41:5A4134303530</setSpec>
      <setSpec>74797065733D636F6E666572656E63655F6974656D</setSpec>
      <setSpec>6469766973696F6E733D3233303030:31313030302F35</setSpec></header>
    <metadata>
      <rioxx xmlns="http://www.rioxx.net/schema/v2.0/rioxx/" xmlns:ali="http://ali.niso.org/2014/ali/1.0" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:rioxxterms="http://docs.rioxx.net/schema/v2.0/rioxxterms/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.rioxx.net/schema/v2.0/rioxx/ http://www.rioxx.net/schema/v2.0/rioxx/rioxx.xsd"><ali:free_to_read/><dc:description>Reviews the development of the Deconstructed Journal academic publishing model. The model was first proposed in something like its present form in 1997 and further developed in 1999. Although not actively promoted elements of the model appear to be emerging spontaneously from the general developments in online academic publishing.</dc:description><dc:format>application/pdf</dc:format><dc:identifier>http://kar.kent.ac.uk/4/1/DorDJEM.pdf</dc:identifier><dc:language>en</dc:language><dc:publisher>Learned Information Europe Ltd</dc:publisher><dc:source>1465-4199</dc:source><dc:subject>ZA</dc:subject><dc:subject>ZA4050</dc:subject><dc:title>The Deconstructed (or Distributed) Journal - an emerging model?</dc:title><rioxxterms:apc>unknown</rioxxterms:apc><rioxxterms:author>Smith, John W.T.</rioxxterms:author><rioxxterms:contributor>Lewis, J.</rioxxterms:contributor><rioxxterms:project funder_name="notused">notused</rioxxterms:project><rioxxterms:publication_date>2004-11</rioxxterms:publication_date><rioxxterms:type>Conference Paper/Proceeding/Abstract</rioxxterms:type><rioxxterms:version>NA</rioxxterms:version></rioxx></metadata></record>

Designed by Paul Walk, built with  Hugo v. 0.26
Copyright © Paul Walk. This website and blog are licensed under a Creative Commons Attribution-ShareAlike 3.0 Unported License